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SCN3B Polyclonal antibody

SCN3B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25907-1-AP

Print datasheet

Synonyms

HSA243396, KIAA1158, SCN3B, SCNB3, Sodium channel subunit beta 3

验证数据展示

  • WB analysis using 25907-1-AP

    WB analysis using 25907-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25907-1-AP (SCN3B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25907-1-AP targets SCN3B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SCN3B fusion protein Ag23201 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sodium channel, voltage-gated, type III, beta
Synonyms HSA243396, KIAA1158, SCN3B, SCNB3, Sodium channel subunit beta 3
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC126265
Gene Symbol SCN3B
Gene ID (NCBI) 55800
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NY72
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The sodium channel is a multi-subunit protein complex composed of a single large α-subunit along with smaller additional β-subunits. There are at least three different β-subunit genes, SCN1b, SCN2b, and SCN3b, all of which are expressed widely in excitable tissues. The bands on Western blots for SCN3b (45-50 kDa) were significantly larger than the predicted molecular weight, which is consistent with the protein being glycosylated. The two bands on the Western blot could be due to different levels of glycosylation or alternative spliced isoforms. (PMID: 11744748)

实验方案

Product Specific Protocols
WB protocol for SCN3B antibody 25907-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols