验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, rat kidney tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
28707-1-AP targets SCNN1A in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | SCNN1A fusion protein Ag30063 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | sodium channel, nonvoltage-gated 1 alpha |
| Synonyms | ENaCa, Amiloride-sensitive sodium channel subunit alpha, Alpha-NaCH, Alpha-ENaC, Alpha NaCH |
| Calculated Molecular Weight | 76 kDa |
| Observed Molecular Weight | 60-70 kDa |
| GenBank Accession Number | BC006526 |
| Gene Symbol | SCNN1A |
| Gene ID (NCBI) | 6337 |
| RRID | AB_2918193 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P37088 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Amiloride-sensitive sodium channel subunit alpha (SCNN1A, also known as Alpha-ENaC and ENaCA) is essential for maintaining sodium homeostasis and is particularly important in the distal nephron and late distal convoluted tubule of the kidney, where aldosterone triggers ENaC activity (PMID: 18086683; 31654198). The ~97 kDa and ~75kDa bands represent the glycosylated and unglycosylated forms of SCNN1A (PMID: 19462466). It also migrated as intact (≈ 75 kDa) and furin-cleaved (≈ 50 kDa) in urinary extracellular vesicles (uEVs) (PMID: 31654198).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SCNN1A antibody 28707-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |