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SCP2/SCPx Polyclonal antibody

SCP2/SCPx Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14397-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

SCP2, NSL-TP, Non-specific lipid-transfer protein, EC:2.3.1.176, EC:2.3.1.16

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA549 cells, BxPC-3 cells, HepG2 cells, NIH/3T3 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inBxPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:300-1:1500
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

14397-1-AP targets SCP2/SCPx in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse, rat
免疫原 SCP2/SCPx fusion protein Ag5895 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sterol carrier protein 2
别名 SCP2, NSL-TP, Non-specific lipid-transfer protein, EC:2.3.1.176, EC:2.3.1.16
计算分子量 59 kDa
观测分子量 44-46 kDa, 58 kDa
GenBank蛋白编号BC067108
基因名称 SCP2
Gene ID (NCBI) 6342
RRIDAB_10644332
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP22307
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SCP2 (Sterol carrier protein-2), also called nonspecific lipid-transfer protein, is thought to play a major role in intracellular lipid transport and metabolism. Mutations of SCP2 have been associated with diseases involving abnormalities in lipid trafficking, such as Zellweger syndrome. The alternative splicing generates 58 kDa SCPx and 13-15 kDa SCP2 proteins, both of which contain a C-terninal SCP2 domain. In addition to the SCP2 domain, SCPx also contains an amino-terminal thiolase domain. The proteolytic cleavage of SCPx occurred when it was imported into peroxisomes, giving rise to a 44-46 kDa thiolase and a 13-15 kDa SCP2. 14397-1-AP antibody recognizes the intact 58 kDa SCPx protein as well as 44-46 kDa thiolase (PMID: 35996156).

实验方案

Product Specific Protocols
WB protocol for SCP2/SCPx antibody 14397-1-APDownload protocol
IHC protocol for SCP2/SCPx antibody 14397-1-APDownload protocol
IF protocol for SCP2/SCPx antibody 14397-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Redox Biol

The mammalian peroxisomal membrane is permeable to both GSH and GSSG - Implications for intraperoxisomal redox homeostasis

Authors - Maria J Ferreira
mouseWB

EMBO Rep

MAPL loss dysregulates bile and liver metabolism in mice

Authors - Vanessa Goyon
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