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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 23006-1-AP (same clone as 23006-1-PBS)

    WB analysis using 23006-1-AP (same clone as 23006-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23006-1-AP (SCP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23006-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 23006-1-AP (same clone as 23006-1-PBS)

    IP experiment of HepG2 using 23006-1-AP (same clone as 23006-1-PBS)

    IP result of anti-SCP2 (IP:23006-1-AP, 4ug; Detection:23006-1-AP 1:500) with HepG2 cells lysate 3600ug. This data was developed using the same antibody clone with 23006-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 23006-1-AP (same clone as 23006-1-PBS)

    IF Staining of HepG2 using 23006-1-AP (same clone as 23006-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 23006-1-AP (SCP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23006-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 23006-1-AP (same clone as 23006-1-PBS)

    WB analysis of HEK-293 using 23006-1-AP (same clone as 23006-1-PBS)

    WB result of SCP2 antibody (23006-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SCP2 transfected HEK-293 cells. This data was developed using the same antibody clone with 23006-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 23006-1-AP (same clone as 23006-1-PBS)

    FC experiment of HepG2 using 23006-1-AP (same clone as 23006-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human SCP2 (23006-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 23006-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SCP2 Polyclonal antibody, PBS Only

SCP2 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 23006-1-PBS

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

23006-1-PBS targets SCP2 in WB, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen SCP2 fusion protein Ag19215 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sterol carrier protein 2
Synonyms
Calculated Molecular Weight 547 aa, 59 kDa
Observed Molecular Weight 13-15 kDa
GenBank Accession NumberBC005911
Gene Symbol SCP2
Gene ID (NCBI) 6342
RRIDAB_2879197
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP22307
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SCP2 (Sterol carrier protein-2), also called nonspecific lipid-transfer protein, is thought to play a major role in intracellular lipid transport and metabolism. Mutations of SCP2 have been associated with diseases involving abnormalities in lipid trafficking, such as Zellweger syndrome. The alternative splicing generates 58 kDa SCPx and 13-15 kDa SCP2 proteins, both of which contain a C-terninal SCP2 domain. In addition to the SCP2 domain, SCPx also contains an amino-terminal thiolase domain. The proteolytic cleavage of SCPx occurred when it was imported into peroxisomes, giving rise to a 44-46 kDa thiolase and a 13-15 kDa SCP2. 23006-1-AP antibody recognizes the 13-15 kDa SCP2 protein.