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验证数据展示

  • WB analysis using 29781-1-AP

    WB analysis using 29781-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 29781-1-AP (SCPEP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse embryo using 29781-1-AP

    IHC staining of mouse embryo using 29781-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 29781-1-AP (SCPEP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse embryo using 29781-1-AP

    IHC staining of mouse embryo using 29781-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 29781-1-AP (SCPEP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SCPEP1 Polyclonal antibody

SCPEP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29781-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IHC, ELISA

别名

HSCP1, RISC, SCP1, SCPEP1, serine carboxypeptidase 1, UNQ265/PRO302

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells
Positive IHC detected inmouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29781-1-AP targets SCPEP1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse
免疫原 SCPEP1 fusion protein Ag31396 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 serine carboxypeptidase 1
别名 HSCP1, RISC, SCP1, SCPEP1, serine carboxypeptidase 1, UNQ265/PRO302
计算分子量 452 aa, 51 kDa
观测分子量33-51 kDa
GenBank蛋白编号BC072405
基因名称 SCPEP1
Gene ID (NCBI) 59342
RRIDAB_2923608
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HB40
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SCPEP1 encodes retinoid-inducible serine carboxypeptidase (PMID:12975309). SCPEP1 and Cathepsin A play roles in regulation of vasoconstriction via inactivation of endothelin-1 (PubMed:24586188). The protein is widely expressed during mouse embryogenesis by immunohistochemical (PubMed:16461364). Scpep1 is proteolytically cleaved to a mature enzyme which is smaller than 51 kDa (PMID:16461364).

实验方案

Product Specific Protocols
WB protocol for SCPEP1 antibody 29781-1-APDownload protocol
IHC protocol for SCPEP1 antibody 29781-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols