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SCRN2 Polyclonal antibody

SCRN2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11485-2-AP

Print datasheet

Synonyms

SCRN2, secernin 2, Ses2

验证数据展示

  • WB analysis of BxPC-3 using 11485-2-AP

    WB analysis of BxPC-3 using 11485-2-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11485-2-AP (SCRN2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 11485-2-AP

    IHC staining of human gliomas using 11485-2-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11485-2-AP (SCRN2 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inBxPC-3 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11485-2-AP targets SCRN2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SCRN2 fusion protein Ag2001 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name secernin 2
Synonyms SCRN2, secernin 2, Ses2
Calculated Molecular Weight 46.6 kDa
Observed Molecular Weight 40-47 kDa
GenBank Accession NumberBC017317
Gene Symbol SCRN2
Gene ID (NCBI) 90507
RRIDAB_2184813
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96FV2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SCRN genes encode protein members of the secernin family including Secernin 1, Secernin 2 and Secernin 3. Function of Secernin 2 and Secernin 3 are so far not well understood. Secernin 1 is a novel 50-kDa cytosolic protein that appears to be involved in the regulation of exocytosis from peritoneal mast cells. Secernin-1 a novel tumor-associated antigen (TAA) and may be a universal marker of different cancer types including gastric cancer, which has been validated by several researches. Measurement of SCRN1 can be used in the early detection of cancer or in the surveillance of patients who undergo surgery.

实验方案

Product Specific Protocols
WB protocol for SCRN2 antibody 11485-2-APDownload protocol
IHC protocol for SCRN2 antibody 11485-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols