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  • Featured Product
  • KD/KO Validated

SCYL3 Polyclonal antibody

SCYL3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 11249-1-AP

Print datasheet

Synonyms

SCY1 like protein 3, Protein-associating with the carboxyl-terminal domain of ezrin, PACE1, PACE 1, Ezrin-binding protein PACE-1

验证数据展示

  • WB analysis of human brain using 11249-1-AP

    WB analysis of human brain using 11249-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11249-1-AP (SCYL3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 11249-1-AP

    IHC staining of human lung cancer using 11249-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11249-1-AP (SCYL3 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 11249-1-AP

    IHC staining of human lung cancer using 11249-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11249-1-AP (SCYL3 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 11249-1-AP

    IF Staining of HeLa using 11249-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11249-1-AP (SCYL3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11249-1-AP targets SCYL3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivitymouse
Immunogen SCYL3 fusion protein Ag1769 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SCY1-like 3 (S. cerevisiae)
Synonyms SCY1 like protein 3, Protein-associating with the carboxyl-terminal domain of ezrin, PACE1, PACE 1, Ezrin-binding protein PACE-1
Calculated Molecular Weight83 kDa
Observed Molecular Weight 83 kDa
GenBank Accession NumberBC014662
Gene Symbol SCYL3
Gene ID (NCBI) 57147
RRIDAB_2071154
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IZE3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SCYL3 antibody 11249-1-APDownload protocol
IHC protocol for SCYL3 antibody 11249-1-APDownload protocol
IF protocol for SCYL3 antibody 11249-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

J Neurosci

Overlapping Role of SCYL1 and SCYL3 in Maintaining Motor Neuron Viability.

Authors - Emin Kuliyev
  • KO Validated
View Article