CD138/Syndecan-1 Monoclonal antibody

CD138/Syndecan-1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 60185-2-Ig

产品说明书

CloneNo. 4H5H5

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, ELISA and More (1)

CD138, CD138/Syndecan-1, SDC, SDC1, SYND1, syndecan, syndecan 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inMCF7 cells
Positive IHC detected inhuman tonsil tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CD138/SDC1 Ready-To-Use IHC Kit. CD138/SDC1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60185-2-Ig targets CD138/Syndecan-1 in WB, IHC, CoIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, CoIP
经测试反应性 human
文献引用反应性human
免疫原 CD138/Syndecan-1 fusion protein Ag0823 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 syndecan 1
别名 CD138, CD138/Syndecan-1, SDC, SDC1, SYND1, syndecan, syndecan 1
计算分子量 32.5 kDa
观测分子量 140 kDa
GenBank蛋白编号BC008765
基因名称 CD138
Gene ID (NCBI) 6382
RRIDAB_11183769
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP18827
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Syndecan-1 (SDC1), also named CD138, is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. SDC1 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas.

实验方案

Product Specific Protocols
WB protocol for CD138/Syndecan-1 antibody 60185-2-IgDownload protocol
IHC protocol for CD138/Syndecan-1 antibody 60185-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,CoIP

Front Cell Dev Biol

The Glypican-1/HGF/C-Met and Glypican-1/VEGF/VEGFR2 Ternary Complexes Regulate Hair Follicle Angiogenesis.

Authors - Charlie Colin-Pierre
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