验证数据展示
经过测试的应用
| Positive WB detected in | MCF7 cells |
| Positive IHC detected in | human tonsil tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy CD138/SDC1 Ready-To-Use IHC Kit. CD138/SDC1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| CoIP | See 1 publications below |
产品信息
60185-2-Ig targets CD138/Syndecan-1 in WB, IHC, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, CoIP |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | CD138/Syndecan-1 fusion protein Ag0823 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | syndecan 1 |
| Synonyms | CD138, CD138/Syndecan-1, SDC, SDC1, SYND1, syndecan, syndecan 1 |
| Calculated Molecular Weight | 32.5 kDa |
| Observed Molecular Weight | 140 kDa |
| GenBank Accession Number | BC008765 |
| Gene Symbol | CD138 |
| Gene ID (NCBI) | 6382 |
| RRID | AB_11183769 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P18827 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Syndecan-1 (SDC1), also named CD138, is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. SDC1 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD138/Syndecan-1 antibody 60185-2-Ig | Download protocol |
| IHC protocol for CD138/Syndecan-1 antibody 60185-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |