验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells |
| Positive IHC detected in | mouse skeletal muscle tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11820-1-AP targets SDC4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | SDC4 fusion protein Ag2388 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | syndecan 4 |
| 别名 | Amphiglycan, Ryudocan core protein, SYND4, syndecan 4, Syndecan-4 |
| 计算分子量 | 22 kDa |
| 观测分子量 | 22-26 kDa |
| GenBank蛋白编号 | BC030805 |
| 基因名称 | SDC4 |
| Gene ID (NCBI) | 6385 |
| RRID | AB_2877797 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P31431 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SDC4, also named as syndecan 4, is a cell surface proteoglycan that bears heparan sulfate, which is involved in normal wound healing. It has been reported that SDC4 may be associated with keratinocyte proliferation and differentiation in the epidermis. SDC4 also plays a key role in the angiogenesis and inflammation. The predicted MW of SDC4 is 22 kDa and 11820-1-AP antibody recognizes a protein around 24-26 kDa and 48-52 kDa (homodimer) which is similar to the papers.(PMID: 20639201, 21949132; 18638267; 30165731 )
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SDC4 antibody 11820-1-AP | Download protocol |
| IHC protocol for SDC4 antibody 11820-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Pharmacol Dioscin Inhibits HSC-T6 Cell Migration via Adjusting SDC-4 Expression: Insights from iTRAQ-Based Quantitative Proteomics. | ||
Cell Death Dis Therapeutic potential of targeting membrane-spanning proteoglycan SDC4 in hepatocellular carcinoma.
| ||
eBioMedicine Atypical E3 ligase ZFP91 promotes small-molecule-induced E2F2 transcription factor degradation for cancer therapy | ||
Front Pediatr Bioinformatics analysis identifies heparan sulfate proteoglycans acting as different progress subtypes of biliary atresia | ||
EBioMedicine Spatial and single-cell colocalisation analysis reveals MDK-mediated immunosuppressive environment with regulatory T cells in colorectal carcinogenesis |
