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SDCCAG8 Monoclonal antibody

SDCCAG8 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2H1A4

Cat no : 66284-1-Ig

Print datasheet

Synonyms

Antigen NY CO 8, BBS16, CCCAP, hCCCAP, HSPC085, NY CO 8, SDCCAG8

验证数据展示

  • WB analysis of HEK-293 using 66284-1-Ig

    WB analysis of HEK-293 using 66284-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66284-1-Ig (SDCCAG8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 66284-1-Ig

    IHC staining of human kidney using 66284-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66284-1-Ig (SDCCAG8 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 66284-1-Ig

    IHC staining of human kidney using 66284-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66284-1-Ig (SDCCAG8 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inHEK-293 cells, THP-1 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66284-1-Ig targets SDCCAG8 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen SDCCAG8 fusion protein Ag4264 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name serologically defined colon cancer antigen 8
Synonyms Antigen NY CO 8, BBS16, CCCAP, hCCCAP, HSPC085, NY CO 8, SDCCAG8
Calculated Molecular Weight 713 aa, 83 kDa
Observed Molecular Weight 83 kDa
GenBank Accession NumberBC032454
Gene Symbol SDCCAG8
Gene ID (NCBI) 10806
RRIDAB_2881667
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ86SQ7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SDCCAG8, also named as CCCAP and NY-CO-8, plays a role in the establishment of cell polarity and epithelial lumen formation. It may play a role in ciliogenesis. Loss of SDCCAG8 function as a cause of a retinal-renal ciliopathy and validates exome capture analysis for broadly heterogeneous single-gene disorders. SDCCAG8 is localized at both centrioles and interacts directly with OFD1 (oral-facial-digital syndrome 1), which is associated with NPHP-RC.

实验方案

Product Specific Protocols
WB protocol for SDCCAG8 antibody 66284-1-IgDownload protocol
IHC protocol for SDCCAG8 antibody 66284-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols