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验证数据展示

  • WB analysis of HEK-293 using 66284-1-Ig

    WB analysis of HEK-293 using 66284-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66284-1-Ig (SDCCAG8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 66284-1-Ig

    IHC staining of human kidney using 66284-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66284-1-Ig (SDCCAG8 Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with SDCCAG8 antibody included
  • IHC staining of human kidney using 66284-1-Ig

    IHC staining of human kidney using 66284-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66284-1-Ig (SDCCAG8 Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with SDCCAG8 antibody included

SDCCAG8 Monoclonal antibody

SDCCAG8 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66284-1-Ig

产品说明书

CloneNo. 2H1A4

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, ELISA

别名

Antigen NY CO 8, BBS16, CCCAP, hCCCAP, HSPC085, NY CO 8, SDCCAG8

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, THP-1 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SDCCAG8 Ready-To-Use IHC Kit. SDCCAG8 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66284-1-Ig targets SDCCAG8 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 SDCCAG8 fusion protein Ag4264 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 serologically defined colon cancer antigen 8
别名 Antigen NY CO 8, BBS16, CCCAP, hCCCAP, HSPC085, NY CO 8, SDCCAG8
计算分子量 713 aa, 83 kDa
观测分子量 83 kDa
GenBank蛋白编号BC032454
基因名称 SDCCAG8
Gene ID (NCBI) 10806
RRIDAB_2881667
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ86SQ7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SDCCAG8, also named as CCCAP and NY-CO-8, plays a role in the establishment of cell polarity and epithelial lumen formation. It may play a role in ciliogenesis. Loss of SDCCAG8 function as a cause of a retinal-renal ciliopathy and validates exome capture analysis for broadly heterogeneous single-gene disorders. SDCCAG8 is localized at both centrioles and interacts directly with OFD1 (oral-facial-digital syndrome 1), which is associated with NPHP-RC.

实验方案

Product Specific Protocols
WB protocol for SDCCAG8 antibody 66284-1-IgDownload protocol
IHC protocol for SDCCAG8 antibody 66284-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols