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验证数据展示

  • WB analysis using 14747-1-AP

    WB analysis using 14747-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14747-1-AP (SDF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 14747-1-AP

    WB analysis using 14747-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14747-1-AP (SDF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 14747-1-AP

    IHC staining of human ovary cancer using 14747-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 14747-1-AP (SDF2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SDF2 Polyclonal antibody

SDF2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14747-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Stromal cell-derived factor 2, stromal cell derived factor 2, SDF-2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HUVEC cells, mouse heart, U2OS cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14747-1-AP targets SDF2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 SDF2 fusion protein Ag6480 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 stromal cell-derived factor 2
别名 Stromal cell-derived factor 2, stromal cell derived factor 2, SDF-2
计算分子量 23 kDa
观测分子量23 kDa
GenBank蛋白编号BC000500
基因名称 SDF2
Gene ID (NCBI) 6388
RRIDAB_2918037
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99470
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SDF-2 is a small protein of 211 amino acids that is ubiquitously expressed in lung, breast, colon, liver and kidney. SDF-2 is localized in the ER and has been reported to get involved in multiple endoplasmic reticulum (ER) functions, including the misfolded protein catabolic process, protein glycosylation, and ER protein quality control.

实验方案

Product Specific Protocols
WB protocol for SDF2 antibody 14747-1-APDownload protocol
IHC protocol for SDF2 antibody 14747-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols