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验证数据展示

  • IHC staining of human liver cancer using 66588-1-Ig (same clone as 66588-1-PBS)

    IHC staining of human liver cancer using 66588-1-Ig (same clone as 66588-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66588-1-Ig (SDHA antibody) at dilution of 1:2000 (under 10x lens). This data was developed using the same antibody clone with 66588-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SDHA antibody included
  • IHC staining of human liver cancer using 66588-1-Ig (same clone as 66588-1-PBS)

    IHC staining of human liver cancer using 66588-1-Ig (same clone as 66588-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66588-1-Ig (SDHA antibody) at dilution of 1:2000 (under 40x lens). This data was developed using the same antibody clone with 66588-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SDHA antibody included
  • WB analysis using 66588-1-Ig (same clone as 66588-1-PBS)

    WB analysis using 66588-1-Ig (same clone as 66588-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66588-1-Ig (SDHA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66588-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66588-1-Ig (same clone as 66588-1-PBS)

    IHC staining of human liver cancer using 66588-1-Ig (same clone as 66588-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66588-1-Ig (SDHA antibody) at dilution of 1:2000 (under 10x lens). This data was developed using the same antibody clone with 66588-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SDHA antibody included
  • IF Staining of HeLa using 66588-1-Ig (same clone as 66588-1-PBS)

    IF Staining of HeLa using 66588-1-Ig (same clone as 66588-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SDHA antibody (66588-1-Ig, Clone: 5E10G12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66588-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50790-1

    Cytometric bead array standard curve of MP50790-1

    Cytometric bead array standard curve of MP50790-1, SDHA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66588-1-PBS. Detection antibody: 66588-2-PBS. Standard:Ag7023. Range: 0.781-200 ng/mL.

  • Cytometric bead array standard curve of MP50790-2

    Cytometric bead array standard curve of MP50790-2

    Cytometric bead array standard curve of MP50790-2, SDHA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66588-1-PBS. Detection antibody: 66588-3-PBS. Standard:Ag7023. Range: 0.781-200 ng/mL.

  • Cytometric bead array standard curve of MP50790-3

    Cytometric bead array standard curve of MP50790-3

    Cytometric bead array standard curve of MP50790-3, SDHA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66588-1-PBS. Detection antibody: 66588-4-PBS. Standard:Ag7023. Range: 0.781-200 ng/mL.

SDHA Monoclonal antibody, PBS Only (Capture)

SDHA Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 66588-1-PBS

产品说明书

CloneNo. 5E10G12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

PPGL5, PGL5, MC2DN1, FP, EC:1.3.5.1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SDHA Ready-To-Use IHC Kit. SDHA primary antibody included.

产品信息

66588-1-PBS targets SDHA as part of a matched antibody pair:

MP50790-1: 66588-1-PBS capture and 66588-2-PBS detection (validated in Cytometric bead array)

MP50790-2: 66588-1-PBS capture and 66588-3-PBS detection (validated in Cytometric bead array)

MP50790-3: 66588-1-PBS capture and 66588-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 SDHA fusion protein Ag7023 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 succinate dehydrogenase complex, subunit A, flavoprotein (Fp)
别名 PPGL5, PGL5, MC2DN1, FP, EC:1.3.5.1
计算分子量 73 kDa
观测分子量 62-73 kDa
GenBank蛋白编号BC001380
基因名称 SDHA
Gene ID (NCBI) 6389
RRIDAB_2881948
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP31040
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SDHA, also named as SDH2, SDHF and Fp, belongs to the FAD-dependent oxidoreductase 2 family and FRD/SDH subfamily. SDHA is the flavoprotein subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDHA has 3 isoforms with the molecular mass of 73, 67 and 57 kDa, and the N-terminal (~5 kDa) can be removed in mature form. It can be detected a band of 68 kDa in cutaneous melanoma(PMID:21698147). SDHA may act as a tumor suppressor.