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SDHD Polyclonal antibody

SDHD Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26519-1-AP

Print datasheet

Synonyms

PGL, CybS, CII-4, CII4, CII 4

验证数据展示

  • IHC staining of human stomach cancer using 26519-1-AP

    IHC staining of human stomach cancer using 26519-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26519-1-AP (SDHD antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SDHD antibody included
  • IF Staining of HepG2 using 26519-1-AP

    IF Staining of HepG2 using 26519-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SDHD antibody (26519-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SDHD Ready-To-Use IHC Kit. SDHD primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26519-1-AP targets SDHD in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen SDHD fusion protein Ag24644 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name succinate dehydrogenase complex, subunit D, integral membrane protein
Synonyms PGL, CybS, CII-4, CII4, CII 4
Calculated Molecular Weight 17 kDa
GenBank Accession NumberBC012603
Gene Symbol SDHD
Gene ID (NCBI) 6392
RRIDAB_2880540
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14521
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for SDHD antibody 26519-1-APDownload protocol
IF protocol for SDHD antibody 26519-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols