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SDS-PAGE 蛋白上样缓冲液(4X)

Cat No. PR20003

浓度

4x

应用

WB

规格价格库存


Product Information

Proteintech生产的 SDS-PAGE 蛋白上样缓冲液是以溴酚蓝为染料,4倍浓缩的蛋白上样缓冲液。本品适用于常规 SDS-PAGE 中蛋白样品的上样。

产品介绍

组分规格
Loading Buffer15 mL


保存条件

-20℃保存,一年有效。

使用方法

1. 室温充分融解 SDS-PAGE上样缓冲液。 

2. 按照 3 : 1比例,混合蛋白样品和 SDS-PAGE上样缓冲液。 

3. 沸水浴加热 5-10分钟,以充分变性蛋白。 

4. 冷却至室温后,直接上样到 SDS-PAGE 胶加样孔内即可。 

注意

1. SDS-PAGE 蛋白上样缓冲液中含 DTT,有轻微刺激性气味,但不含剧毒的巯基乙醇。 

2. SDS-PAGE 蛋白上样缓冲液必须完全融解后再使用,可用37℃以下温水融解。 

3. 为了您的安全和健康,请穿实验服并戴一次性手套操作。

4. 为取得最佳的使用效果,尽量避免过多的反复冻融,可以适当分装使用。

Publications

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Yunnan Baiyao regulates Th17/Treg cell homeostasis to inhibit periodontitis through the miR-155-5p/ATG5 pathway

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Long Non-Coding RNA-Cardiac-Inducing RNA 6 Mediates Repair of Infarcted Hearts by Inducing Mesenchymal Stem Cell Differentiation into Cardiogenic Cells through Cyclin-Dependent Kinase 1

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