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SDSL Polyclonal antibody

SDSL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16242-1-AP

Print datasheet

Synonyms

L-serine dehydratase/L-threonine deaminase, L-serine deaminase, L threonine dehydratase, L serine deaminase, EC:4.3.1.19

验证数据展示

  • WB analysis of HepG2 using 16242-1-AP

    WB analysis of HepG2 using 16242-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16242-1-AP (SDSL antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 16242-1-AP

    IF Staining of HepG2 using 16242-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16242-1-AP (SDSL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human liver using 16242-1-AP

    IHC staining of human liver using 16242-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16242-1-AP (SDSL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SDSL antibody included
  • IHC staining of human liver using 16242-1-AP

    IHC staining of human liver using 16242-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16242-1-AP (SDSL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SDSL antibody included
  • IHC staining of mouse liver using 16242-1-AP

    IHC staining of mouse liver using 16242-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16242-1-AP (SDSL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SDSL antibody included
  • IHC staining of mouse liver using 16242-1-AP

    IHC staining of mouse liver using 16242-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16242-1-AP (SDSL antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SDSL antibody included

经过测试的应用

Positive WB detected inHepG2 cells, human colon tissue, human liver tissue, mouse liver tissue, mouse lung tissue
Positive IHC detected inhuman liver tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SDSL Ready-To-Use IHC Kit. SDSL primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16242-1-AP targets SDSL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen SDSL fusion protein Ag9114 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name serine dehydratase-like
Synonyms L-serine dehydratase/L-threonine deaminase, L-serine deaminase, L threonine dehydratase, L serine deaminase, EC:4.3.1.19
Calculated Molecular Weight 329 aa, 35 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC009849
Gene Symbol SDSL
Gene ID (NCBI) 113675
RRIDAB_2254163
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96GA7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SDSL antibody 16242-1-APDownload protocol
IHC protocol for SDSL antibody 16242-1-APDownload protocol
IF protocol for SDSL antibody 16242-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols