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验证数据展示

  • IHC staining of human liver cancer using 67379-1-Ig

    IHC staining of human liver cancer using 67379-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67379-1-Ig (SEC11A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 67379-1-Ig

    IHC staining of human liver cancer using 67379-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67379-1-Ig (SEC11A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 67379-1-Ig

    WB analysis using 67379-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67379-1-Ig (SEC11A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

SEC11A Monoclonal antibody

SEC11A Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67379-1-Ig

产品说明书

CloneNo. 2B11G12

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Mouse, Rat

应用

WB, IHC, ELISA

别名

1810012E07Rik, Endopeptidase SP18, SEC11 homolog A, SEC11 like protein 1, SEC11A, SEC11L1, sid2895, SPase 18 kDa subunit, SPC18, SPCS4A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HSC-T6 cells, HepG2 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67379-1-Ig targets SEC11A in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 SEC11A fusion protein Ag6610 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 SEC11 homolog A (S. cerevisiae)
别名 1810012E07Rik, Endopeptidase SP18, SEC11 homolog A, SEC11 like protein 1, SEC11A, SEC11L1, sid2895, SPase 18 kDa subunit, SPC18, SPCS4A
计算分子量 21 kDa
观测分子量 17-21 kDa
GenBank蛋白编号BC000359
基因名称 SEC11A
Gene ID (NCBI) 23478
RRIDAB_2882626
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP67812
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

SEC11A(Signal peptidase complex catalytic subunit SEC11A) is also named as SEC11L1, SPC18, SPCS4A and belongs to the peptidase S26B family. It is a component of the microsomal signal peptidase complex which removes signal peptides from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. SEC11A has some isoforms with the molecular mass of 17-21 kDa.

实验方案

Product Specific Protocols
WB protocol for SEC11A antibody 67379-1-IgDownload protocol
IHC protocol for SEC11A antibody 67379-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols