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  • Featured Product
  • KD/KO Validated

SEC14L1 Polyclonal antibody

SEC14L1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 25541-1-AP

Print datasheet

Synonyms

SEC14-like protein 1, SEC14L

验证数据展示

  • WB analysis using 25541-1-AP

    WB analysis using 25541-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25541-1-AP (SEC14L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 25541-1-AP

    IP experiment of HEK-293 using 25541-1-AP

    IP result of anti-SEC14L1 (IP:25541-1-AP, 4ug; Detection:25541-1-AP 1:500) with HEK-293 cells lysate 1480 ug.

  • IF Staining of A431 using 25541-1-AP

    IF Staining of A431 using 25541-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using SEC14L1 antibody (25541-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, Y79 cells, HeLa cells
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25541-1-AP targets SEC14L1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen SEC14L1 fusion protein Ag22233 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SEC14-like 1 (S. cerevisiae)
Synonyms SEC14-like protein 1, SEC14L
Calculated Molecular Weight 719 aa, 82 kDa
Observed Molecular Weight 81 kDa, 77 kDa
GenBank Accession NumberBC142979
Gene Symbol SEC14L1
Gene ID (NCBI) 6397
RRIDAB_2880125
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92503
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SEC14L1 (SEC14-like protein 1) is a member of SEC14 family in mammals with the potential to control local phospholipid content in membranes. SEC14L1 may act as a phospholipid transfer protein. It has also been reported that SEC14L1 functions as a novel negative regulator of RIG-I-mediated antiviral signaling and is involved in innate immunity (PMID: 17092608; 23843640). SEC14L1 has three isoforms with the molecular mass of 81.2kDa, 81.8kDa and 77.1kDa.

实验方案

Product Specific Protocols
WB protocol for SEC14L1 antibody 25541-1-APDownload protocol
IF protocol for SEC14L1 antibody 25541-1-APDownload protocol
IP protocol for SEC14L1 antibody 25541-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Elife

The ORP9-ORP11 dimer promotes sphingomyelin synthesis

Authors - Birol Cabukusta
  • KO Validated
View Article
humanWB

Mol Cell

mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway

Authors - Sang Ah Yi
View Article