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验证数据展示

  • WB analysis using 17245-1-AP

    WB analysis using 17245-1-AP

    mouse pancreas tissue was subjected to SDS PAGE followed by western blot with 17245-1-AP (SEC16B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 17245-1-AP

    IHC staining of human pancreas cancer using 17245-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17245-1-AP (SEC16B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SEC16B Polyclonal antibody

SEC16B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17245-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

KIAA1928, LZTR2, PGPR p117, RGPR, RGPR p117, SEC16 homolog B, SEC16B, SEC16S

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse pancreas tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17245-1-AP targets SEC16B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 SEC16B fusion protein Ag11173 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SEC16 homolog B (S. cerevisiae)
别名 KIAA1928, LZTR2, PGPR p117, RGPR, RGPR p117, SEC16 homolog B, SEC16B, SEC16S
计算分子量 71 kDa, 117 kDa
观测分子量 60-70 kDa
GenBank蛋白编号BC009106
基因名称 SEC16B
Gene ID (NCBI) 89866
RRIDAB_3085519
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96JE7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SEC16B also known as LZTR2, RGPR-p117, is a mammalian homolog of S. cerevisiae Sec16 that is required for organization of transitional endoplasmic reticulum (ER) sites and protein export (PMID: 17192411). SEC16B regulates the transport of peroxisomal biogenesis factors PEX3 and PEX16 from the ER to peroxisomes (PMID:21768384). SEC16B may be involved in human obesity to possess an association between single nucleotide polymorphisms and different measures of obesity (PMID: 28413634).

实验方案

Product Specific Protocols
WB protocol for SEC16B antibody 17245-1-APDownload protocol
IHC protocol for SEC16B antibody 17245-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols