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SEC22C Polyclonal antibody

SEC22C Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28078-1-AP

Print datasheet

Synonyms

DKFZp761F2321, SEC22C, SEC22L3

验证数据展示

  • WB analysis using 28078-1-AP

    WB analysis using 28078-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 28078-1-AP (SEC22C antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 28078-1-AP

    IHC staining of human liver cancer using 28078-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28078-1-AP (SEC22C antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 28078-1-AP

    IHC staining of human liver cancer using 28078-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28078-1-AP (SEC22C antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inK-562 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28078-1-AP targets SEC22C in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen SEC22C fusion protein Ag27896 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SEC22 vesicle trafficking protein homolog C (S. cerevisiae)
Synonyms DKFZp761F2321, SEC22C, SEC22L3
Calculated Molecular Weight 34 kDa
Observed Molecular Weight34 kDa
GenBank Accession NumberBC018437
Gene Symbol SEC22C
Gene ID (NCBI) 9117
RRIDAB_2881053
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BRL7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SEC22C antibody 28078-1-APDownload protocol
IHC protocol for SEC22C antibody 28078-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols