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验证数据展示

  • WB analysis of mouse brain using 12751-1-AP (same clone as 12751-1-PBS)

    WB analysis of mouse brain using 12751-1-AP (same clone as 12751-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12751-1-AP (SEC5/EXOC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12751-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 12751-1-AP (same clone as 12751-1-PBS)

    IP experiment of mouse brain using 12751-1-AP (same clone as 12751-1-PBS)

    IP result of anti-SEC5/EXOC2 (IP:12751-1-AP, 5ug; Detection:12751-1-AP 1:500) with mouse brain tissue lysate 3000ug. This data was developed using the same antibody clone with 12751-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 12751-1-AP (same clone as 12751-1-PBS)

    IHC staining of human breast cancer using 12751-1-AP (same clone as 12751-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 12751-1-AP (SEC5/EXOC2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12751-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 12751-1-AP (same clone as 12751-1-PBS)

    IF Staining of HeLa using 12751-1-AP (same clone as 12751-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 12751-1-AP (SEC5/EXOC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12751-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

SEC5/EXOC2 Polyclonal antibody, PBS Only

SEC5/EXOC2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12751-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

12751-1-PBS targets SEC5/EXOC2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SEC5/EXOC2 fusion protein Ag3147 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 exocyst complex component 2
别名
计算分子量 924 aa, 104 kDa
观测分子量 95-100 kDa
GenBank蛋白编号BC016918
基因名称 SEC5
Gene ID (NCBI) 55770
RRIDAB_2262528
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96KP1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EXOC2 (exocyst complex component 2), also known as SEC5 and SEC5L1, is a component of the exocyst complex, and is required to mediate RalB-dependent survival signals in transformed cells. The exocyst complex, composed of eight evolutionarily conserved subunits (SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70, and EXO84), is involved in tethering post-Golgi secretory vesicles to specific plasma membrane domains. The gene of EXOC2 maps to chromosome 6p25.3, and encodes a 924-amino acid protein with an experimentally determined molecular mass of 95-100 kDa. EXOC2 mRNA is widely expressed with highest levels in brain and placenta.