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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
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0
Conjugate
0

验证数据展示

  • WB analysis using 51020-2-AP (same clone as 51020-2-PBS)

    WB analysis using 51020-2-AP (same clone as 51020-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 51020-2-AP (SEC61B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse pancreas using 51020-2-AP (same clone as 51020-2-PBS)

    IHC staining of mouse pancreas using 51020-2-AP (same clone as 51020-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 51020-2-AP (SEC61B antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse pancreas using 51020-2-AP (same clone as 51020-2-PBS)

    IHC staining of mouse pancreas using 51020-2-AP (same clone as 51020-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 51020-2-AP (SEC61B antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

  • IF Staining of mouse pancreas using 51020-2-AP (same clone as 51020-2-PBS)

    IF Staining of mouse pancreas using 51020-2-AP (same clone as 51020-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using SEC61B antibody (51020-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

  • IF Staining of mouse pancreas using 51020-2-AP (same clone as 51020-2-PBS)

    IF Staining of mouse pancreas using 51020-2-AP (same clone as 51020-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using SEC61B antibody (51020-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 51020-2-AP (same clone as 51020-2-PBS)

    IF Staining of HepG2 using 51020-2-AP (same clone as 51020-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 51020-2-AP (SEC61B antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

SEC61B Polyclonal antibody, PBS Only

SEC61B Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 51020-2-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

51020-2-PBS targets SEC61B in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Sec61 beta subunit
别名
计算分子量 10 kDa
观测分子量 10-15 kDa
GenBank蛋白编号BC001734
基因名称 SEC61B
Gene ID (NCBI) 10952
RRIDAB_2186413
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP60468
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The heteromeric SEC61 complex is composed of alpha, beta and gamma subunits. Oligomers of the SEC61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane (PMID: 10212142). SEC61B is the beta subunit of the SEC61 complex. It has been reported that SEC61B facilitates cotranslational protein transport and interacts with the signal peptidase during translocation (PMID: 9585408).