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SEC61B-Specific Polyclonal antibody, PBS Only

SEC61B-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14846-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 14846-1-AP (same clone as 14846-1-PBS)

    WB analysis using 14846-1-AP (same clone as 14846-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14846-1-AP (SEC61B-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 14846-1-AP (same clone as 14846-1-PBS)

    IF Staining of HeLa using 14846-1-AP (same clone as 14846-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using SEC61B-Specific antibody (14846-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 14846-1-AP (same clone as 14846-1-PBS)

    IHC staining of mouse liver using 14846-1-AP (same clone as 14846-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14846-1-AP (SEC61B-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 14846-1-AP (same clone as 14846-1-PBS)

    IHC staining of mouse liver using 14846-1-AP (same clone as 14846-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14846-1-AP (SEC61B-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse pancreas using 14846-1-AP (same clone as 14846-1-PBS)

    IF Staining of mouse pancreas using 14846-1-AP (same clone as 14846-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using SEC61B-Specific antibody (14846-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.

产品信息

14846-1-PBS targets SEC61B-Specific in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Sec61 beta subunit
Synonyms
Calculated Molecular Weight 10 kDa
Observed Molecular Weight 10-15 kDa
GenBank Accession NumberNM_006808
Gene Symbol SEC61B
Gene ID (NCBI) 10952
RRIDAB_2270168
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP60468
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The heteromeric SEC61 complex is composed of alpha, beta and gamma subunits. Oligomers of the SEC61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane (PMID: 10212142). SEC61B is the beta subunit of the SEC61 complex. It has been reported that SEC61B facilitates cotranslational protein transport and interacts with the signal peptidase during translocation (PMID: 9585408).