验证数据展示
经过测试的应用
| Positive WB detected in | EC109 cells, HEK-293 cells, DC2.4 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11147-2-AP targets SEC61G in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IP, IF, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | SEC61G fusion protein Ag1624 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Sec61 gamma subunit |
| 别名 | Sec61 gamma subunit, SEC61G, SSS1 |
| 计算分子量 | 7.7 kDa |
| 观测分子量 | 7.7 kDa |
| GenBank蛋白编号 | BC009480 |
| 基因名称 | SEC61G |
| Gene ID (NCBI) | 23480 |
| RRID | AB_2254450 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P60059 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SEC61G is a subunit of the SEC61 complex, which also contains alpha (SEC61A) and beta (SEC61B) subunits. The SEC61 complex is the central component of the protein translocation apparatus on the endoplasmic reticulum membrane. The gene of SEC61G maps to chromosome 7p11.2, and encodes a 68-amino acid single-pass membrane protein. This antibody can recognize endogenous SEC61G that migrates at a molecular mass of ~8 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SEC61G antibody 11147-2-AP | Download protocol |
| IHC protocol for SEC61G antibody 11147-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Immunity The translocon protein sec61 mediates antigen transport from endosomes in the cytosol for cross-presentation to CD8(+) T cells.
| ||
Proc Natl Acad Sci U S A Ablation of cholesterol biosynthesis in neural stem cells increases their VEGF expression and angiogenesis but causes neuron apoptosis. | ||
Proc Natl Acad Sci U S A A scalable strategy for high-throughput GFP tagging of endogenous human proteins. | ||
Cancer Res Glioblastoma proto-oncogene SEC61gamma is required for tumor cell survival and response to endoplasmic reticulum stress.
| ||
Elife An allosteric Sec61 inhibitor traps nascent transmembrane helices at the lateral gate. | ||
J Pathol Genome-wide analysis of DNA copy number alterations and gene expression in gastric cancer. |
