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SEC61 Alpha Polyclonal antibody

SEC61 Alpha Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IF/ICC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 24935-1-AP

Print datasheet

Synonyms

Sec61 alpha 2, SEC61A, SEC61A2, SEC61alpha

验证数据展示

  • WB analysis of mouse brain using 24935-1-AP

    WB analysis of mouse brain using 24935-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24935-1-AP (SEC61alpha antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 24935-1-AP

    IF Staining of HepG2 using 24935-1-AP

    Immunofluorescent analysis of HepG2 cells using 24935-1-AP (SEC61alpha antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse brain tissue, HEK-293 cells, U2OS cells, mouse liver tissue
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24935-1-AP is SEC61 Alpha Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, WB, ELISA Application Description
Cited ApplicationsIF, CoIP
Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Immunogen SEC61 Alpha fusion protein Ag20779 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Sec61 alpha 2 subunit (S. cerevisiae)
Synonyms Sec61 alpha 2, SEC61A, SEC61A2, SEC61alpha
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC026179
Gene Symbol SEC61A2
Gene ID (NCBI) 55176
RRIDAB_2879807
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H9S3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The SEC61 complex is a major component of the endoplasmic reticulum (ER) translocation site (PMID: 7720858). This complex is composed of three subunits: SEC61alpha, SEC61beta and SEC61gamma. SEC61alpha is a polytopic integral membrane protein. It is a core component of the ER translocation complex, playing a crucial role in the insertion of secretory and membrane polypeptides into the ER (PMID: 9512475).

实验方案

Product Specific Protocols
WB protocol for SEC61 Alpha antibody 24935-1-APDownload protocol
IF protocol for SEC61 Alpha antibody 24935-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Sci Adv

Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor.

Authors - Yuan Wang
View Article
ratCoIP

iScience

The endoplasmic reticulum membrane complex promotes proteostasis of GABAA receptors

Authors - Angela L. Whittsette
View Article