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验证数据展示

  • WB analysis of mouse brain using 24935-1-AP

    WB analysis of mouse brain using 24935-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24935-1-AP (SEC61alpha antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

SEC61 Alpha Polyclonal antibody

SEC61 Alpha Polyclonal Antibody for WB, ELISA
Cat No. 24935-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, ELISA and More (2)

别名

SEC61A2, Sec61 alpha-2, Protein transport protein Sec61 subunit alpha isoform 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, HEK-293 cells, U2OS cells, mouse liver tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24935-1-AP targets SEC61 Alpha in WB, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
文献引用应用IF, CoIP
经测试反应性 human, mouse
文献引用反应性human, rat
免疫原 SEC61 Alpha fusion protein Ag20779 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Sec61 alpha 2 subunit (S. cerevisiae)
别名 SEC61A2, Sec61 alpha-2, Protein transport protein Sec61 subunit alpha isoform 2
计算分子量 52 kDa
观测分子量 52 kDa
GenBank蛋白编号BC026179
基因名称 SEC61 Alpha
Gene ID (NCBI) 55176
RRIDAB_2879807
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H9S3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The SEC61 complex is a major component of the endoplasmic reticulum (ER) translocation site (PMID: 7720858). This complex is composed of three subunits: SEC61alpha, SEC61beta and SEC61gamma. SEC61alpha is a polytopic integral membrane protein. It is a core component of the ER translocation complex, playing a crucial role in the insertion of secretory and membrane polypeptides into the ER (PMID: 9512475).

实验方案

Product Specific Protocols
WB protocol for SEC61 Alpha antibody 24935-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Sci Adv

Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor.

Authors - Yuan Wang
View Article
ratCoIP

iScience

The endoplasmic reticulum membrane complex promotes proteostasis of GABAA receptors

Authors - Angela L. Whittsette
View Article