验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HEK-293 cells, PC-3 cells |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human liver tissue, mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse kidney tissue |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13978-1-AP targets SEC63 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | SEC63 fusion protein Ag5021 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SEC63 homolog (S. cerevisiae) |
| 别名 | Translocation protein SEC63 homolog, SEC63L, DNAJC23, DnaJ homolog subfamily C member 23 |
| 计算分子量 | 88 kDa |
| 观测分子量 | 83-90 kDa |
| GenBank蛋白编号 | BC047221 |
| 基因名称 | SEC63 |
| Gene ID (NCBI) | 11231 |
| RRID | AB_2186546 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UGP8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SEC63 is an integral ER membrane protein. SEC63 and SEC62 form a dimeric complex and play a central role in the translocation of nascent and newly synthesized precursor polypeptides into the ER. Mutations in the gene encoding SEC63 cause polycystic liver disease in humans. The intact SEC63 protein is usually detected around 83-90 kDa, while smaller cleavage products can be observed by autophagy proteolysis (PMID: 25052096)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SEC63 antibody 13978-1-AP | Download protocol |
| IHC protocol for SEC63 antibody 13978-1-AP | Download protocol |
| IF protocol for SEC63 antibody 13978-1-AP | Download protocol |
| IP protocol for SEC63 antibody 13978-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Cotranslational Stabilization of Sec62/63 within the ER Sec61 Translocon Is Controlled by Distinct Substrate-Driven Translocation Events. | ||
J Am Soc Nephrol Essential Role of X-Box Binding Protein-1 during Endoplasmic Reticulum Stress in Podocytes.
| ||
J Biol Chem ER entry pathway and glycosylation of GPI-anchored proteins are determined by N-terminal signal sequence and C-terminal GPI-attachment sequence
| ||
Neural Regen Res AAV2-PDE6B restores retinal structure and function in the retinal degeneration 10 mouse model of retinitis pigmentosa by promoting phototransduction and inhibiting apoptosis |
