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验证数据展示

  • WB analysis using 28665-1-AP

    WB analysis using 28665-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28665-1-AP (SEC63 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse pancreas using 28665-1-AP

    IHC staining of mouse pancreas using 28665-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 28665-1-AP (SEC63 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse pancreas using 28665-1-AP

    IHC staining of mouse pancreas using 28665-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 28665-1-AP (SEC63 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 28665-1-AP

    IF Staining of HEK-293 using 28665-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SEC63 antibody (28665-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

SEC63 Polyclonal antibody

SEC63 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 28665-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

ERdj2, PRO2507, SEC63, SEC63 homolog (S. cerevisiae), SEC63L

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells
Positive IHC detected inmouse pancreas tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28665-1-AP targets SEC63 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse
免疫原 SEC63 fusion protein Ag30213 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SEC63 homolog (S. cerevisiae)
别名 ERdj2, PRO2507, SEC63, SEC63 homolog (S. cerevisiae), SEC63L
计算分子量 88 kDa
观测分子量 90 kDa
GenBank蛋白编号BC047221
基因名称 SEC63
Gene ID (NCBI) 11231
RRIDAB_2881189
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UGP8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SEC63 is a integral ER membrane protein. SEC63 and SEC62 form a dimeric complex and play a central role in translocation of nascent and newly synthesized precursor polypeptides into the ER. Mutations in the gene encoding SEC63 cause polycystic liver disease in humans. The intact SEC63 protein is usually detected around 83-90 kDa, while smaller cleavage products can be observed by autophagy proteolysis (PMID: 25052096)

实验方案

Product Specific Protocols
WB protocol for SEC63 antibody 28665-1-APDownload protocol
IHC protocol for SEC63 antibody 28665-1-APDownload protocol
IF protocol for SEC63 antibody 28665-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols