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SEH1L Polyclonal antibody

SEH1L Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15565-1-AP

Print datasheet

Synonyms

SEH1, SEC13-like protein, SEC13L, Nup107-160 subcomplex subunit SEH1, Nucleoporin SEH1

验证数据展示

  • WB analysis using 15565-1-AP

    WB analysis using 15565-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15565-1-AP (SEH1L antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 15565-1-AP

    IF Staining of A549 using 15565-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using SEH1L antibody (15565-1-AP) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IF/ICC detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15565-1-AP targets SEH1L in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen SEH1L fusion protein Ag7795 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SEH1-like (S. cerevisiae)
Synonyms SEH1, SEC13-like protein, SEC13L, Nup107-160 subcomplex subunit SEH1, Nucleoporin SEH1
Calculated Molecular Weight 39.6 kDa
Observed Molecular Weight36-39 kDa
GenBank Accession NumberBC012430
Gene Symbol SEH1L
Gene ID (NCBI) 81929
RRIDAB_3085466
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96EE3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SEH1L antibody 15565-1-APDownload protocol
IF protocol for SEH1L antibody 15565-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Apoptosis

SEH1L siliencing induces ferroptosis and suppresses hepatocellular carcinoma progression via ATF3/HMOX1/GPX4 axis

Authors - Ziyang Feng
View Article