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验证数据展示

  • WB analysis using 29801-1-AP

    WB analysis using 29801-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29801-1-AP (SEL1L antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 29801-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 29801-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 29801-1-AP (SEL1L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 29801-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 29801-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 29801-1-AP (SEL1L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SEL1L Polyclonal antibody

SEL1L Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29801-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Protein sel 1 homolog 1, Protein sel-1 homolog 1, Sel 1L, Sel-1L, Suppressor of lin-12-like protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, BxPC-3 cells, HEK-293T cells, mouse kidney tissue, rat liver tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29801-1-AP targets SEL1L in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SEL1L fusion protein Ag32178 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sel-1 suppressor of lin-12-like (C. elegans)
别名 Protein sel 1 homolog 1, Protein sel-1 homolog 1, Sel 1L, Sel-1L, Suppressor of lin-12-like protein 1
计算分子量 89 kDa
观测分子量89 kDa
GenBank蛋白编号NM_005065
基因名称 SEL1L
Gene ID (NCBI) 6400
RRIDAB_3086166
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UBV2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SEL1L (Suppressor Enhancer Lin-12 Like) is an endoplasmic reticulum-resident transmembrane protein that plays a critical role in collagen metabolism. It has been identified as an intracellular rheostat controlling collagen turnover, which is the balance between collagen synthesis and degradation. The protein's luminal face contains a Fibronectin-2 (FN2) domain, which is known to bind collagen. SEL1L functions as a sensor of collagen biosynthesis and is necessary for the upregulation of MRC2 (mannose receptor, C-type 2), a collagen endocytic receptor that mediates the clearance of collagen.

实验方案

Product Specific Protocols
WB protocol for SEL1L antibody 29801-1-APDownload protocol
IHC protocol for SEL1L antibody 29801-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols