E-selectin/CD62E Polyclonal antibody

E-selectin/CD62E Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20894-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA and More (1)

E-selectin / CD62E, CD62E, E-selectin, CD62 antigen-like family member E, ELAM1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHUVEC cells
Positive IHC detected inhuman appendicitis tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20894-1-AP targets E-selectin/CD62E in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human
免疫原 E-selectin/CD62E fusion protein Ag14944 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 selectin E
别名 E-selectin / CD62E, CD62E, E-selectin, CD62 antigen-like family member E, ELAM1
计算分子量 610 aa, 67 kDa
观测分子量115 kDa
GenBank蛋白编号BC142711
基因名称 CD62E
Gene ID (NCBI) 6401
RRIDAB_10755287
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP16581
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

E-selectin, also known as ELAM-1 or CD62E, is a member of the selectin family of adhesion molecules that also includes L-selectin and P-selectin. E-selectin is an inducible endothelial cell surface molecule. Its expression on endothelial cells is transcriptionally upregulated by various proinflammatory substances such as IL-1, TNFα and lipopolysaccharide (LPS). Besides, it can be secreted by endothelial cells, and can be detected in a soluble form (sE-selectin) in serum. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. (PMID: 2827173; 1382417; 8943958)

实验方案

Product Specific Protocols
WB protocol for E-selectin/CD62E antibody 20894-1-APDownload protocol
IHC protocol for E-selectin/CD62E antibody 20894-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Adv Sci (Weinh)

Astrocyte-Derived Extracellular Vesicular miR-143-3p Dampens Autophagic Degradation of Endothelial Adhesion Molecules and Promotes Neutrophil Transendothelial Migration after Acute Brain Injury

Authors - Xun Wu
humanWB

Sci Adv

The support of genetic evidence for cardiovascular risk induced by antineoplastic drugs.

Authors - Hui Cui
humanIF

Adv Healthc Mater

Boosting Tumor Accumulation of Phthalocyanine through Sialylation Engineering for Superior Cancer Phototherapy

Authors - Jian Li
WB

ACS Appl Mater Interfaces

Inflammation-Driven Nanohitchhiker Enhances Postoperative Immunotherapy by Alleviating Prostaglandin E2-Mediated Immunosuppression

Authors - Yingke Liu
humanIF

Biomater Sci

An E-selectin targeting and MMP-2-responsive dextran-curcumin polymeric prodrug for targeted therapy of acute kidney injury

Authors - Jing-Bo Hu
humanWB

Genomics

MIN score predicts primary response to infliximab/adalimumab and vedolizumab therapy in patients with inflammatory bowel diseases.

Authors - Yuan Shi
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