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验证数据展示

  • WB analysis using 27449-1-AP

    WB analysis using 27449-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27449-1-AP (SELENBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 27449-1-AP

    IHC staining of human liver cancer using 27449-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27449-1-AP (SELENBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SELENBP1 antibody included
  • IHC staining of human liver cancer using 27449-1-AP

    IHC staining of human liver cancer using 27449-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27449-1-AP (SELENBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SELENBP1 antibody included

SELENBP1 Polyclonal antibody

SELENBP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27449-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Methanethiol oxidase, LPSB, hSP56, hSBP, EC:1.8.3.4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse liver tissue, L02 cells, MCF-7 cells
Positive IHC detected inhuman liver cancer tissue, human colon tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SELENBP1 Ready-To-Use IHC Kit. SELENBP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27449-1-AP targets SELENBP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SELENBP1 fusion protein Ag24453 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 selenium binding protein 1
别名 Methanethiol oxidase, LPSB, hSP56, hSBP, EC:1.8.3.4
计算分子量 52 kDa
观测分子量 56 kDa
GenBank蛋白编号BC009084
基因名称 SELENBP1
Gene ID (NCBI) 8991
RRIDAB_2880872
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13228
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SELENBP1 (selenium binding protein 1,SBP1), also known as LPSB or SP56, is a 472 amino acid peripheral membrane protein that binds selenium covalently. Selenium, an essential trace element, has been shown to have an anti-cancer effect. Many reports have described a relationship between insufficient selenium intake and increased risk of cancer. Moreover, the expression of SELENBP1 has been shown to be decreased in several tumors including cancers of the prostate, lungs, colon, and ovary (PMID: 21143902, 20332323, 18272210). SELENBP1 has four isoforms with the molecular mass of 56kD, 52kD and 45kD.

实验方案

Product Specific Protocols
WB protocol for SELENBP1 antibody 27449-1-APDownload protocol
IHC protocol for SELENBP1 antibody 27449-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols