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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 27836-1-AP (same clone as 27836-1-PBS)

    WB analysis using 27836-1-AP (same clone as 27836-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27836-1-AP (SEMA3A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27836-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 27836-1-AP (same clone as 27836-1-PBS)

    IF Staining of HeLa using 27836-1-AP (same clone as 27836-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SEMA3A antibody (27836-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 27836-1-PBS in a different storage buffer formulation.

SEMA3A Polyclonal antibody, PBS Only

SEMA3A Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 27836-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

27836-1-PBS targets SEMA3A in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 SEMA3A fusion protein Ag27173 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
别名
计算分子量 771 aa, 89 kDa
观测分子量 89 and 65 kDa
GenBank蛋白编号BC111416
基因名称 SEMA3A
Gene ID (NCBI) 10371
RRIDAB_2880989
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14563
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SEMA3A (Semaphorin 3A), a class-3 semaphorin signaling protein, is involved in cell signaling with PlexinA1 and Neuropilin-1 (NRP1) receptors and it is responsible for recruiting dendritic cells into lymphatics. It's widely expressed in different tissues (PMID:10766232). SEMA3A has been shown to exert osteoprotective effects, characterized by increasing bone formation while inhibiting the activation of osteoclast precursor cells (PMID:36598593). However, SEMA3A also modulates the immune system. SEMA3A expression in a myocardial infarct area enhances macrophage polarization and inhibits monocyte migration into the lesion area (PMID:28540528).