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验证数据展示

  • WB analysis using 83579-5-RR

    WB analysis using 83579-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83579-5-RR (SEMA3A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 83579-5-RR

    IHC staining of human ovary cancer using 83579-5-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83579-5-RR (SEMA3A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 83579-5-RR

    IHC staining of mouse heart using 83579-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83579-5-RR (SEMA3A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83579-5-RR

    Affinity and Kinetic Characterization of 83579-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83579-5-RR against Human SEMA3A were performed. The affinity constant is 0.228 nM.

SEMA3A Recombinant antibody

SEMA3A Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83579-5-RR

产品说明书

CloneNo. 240509E3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

SEMA1, Sema III, Hsema III, Hsema I, coll 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSKOV-3 cells, PC-12 cells, C6 cells
Positive IHC detected inhuman ovary cancer tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83579-5-RR targets SEMA3A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SEMA3A fusion protein Ag27173 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
别名 SEMA1, Sema III, Hsema III, Hsema I, coll 1
计算分子量 771 aa, 89 kDa
观测分子量89 kDa
GenBank蛋白编号BC111416
基因名称 SEMA3A
Gene ID (NCBI) 10371
RRIDAB_3671192
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ14563
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SEMA3A (Semaphorin 3A), a class-3 semaphorin signaling protein, is involved in cell signaling with PlexinA1 and Neuropilin-1 (NRP1) receptors and it is responsible for recruiting dendritic cells into lymphatics. It's widely expressed in different tissues (PMID:10766232). SEMA3A has been shown to exert osteoprotective effects, characterized by increasing bone formation while inhibiting the activation of osteoclast precursor cells (PMID:36598593). However, SEMA3A also modulates the immune system. SEMA3A expression in a myocardial infarct area enhances macrophage polarization and inhibits monocyte migration into the lesion area (PMID:28540528).

实验方案

Product Specific Protocols
WB protocol for SEMA3A antibody 83579-5-RRDownload protocol
IHC protocol for SEMA3A antibody 83579-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols