验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue, mouse brain tissue |
| Positive IP detected in | human placenta tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IP | See 1 publications below |
产品信息
18070-1-AP targets SEMA7A in WB, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | SEMA7A fusion protein Ag12707 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group) |
| 别名 | CD108, CDw108, H SEMA K1, H Sema L, JMH, JMH blood group antigen, Sema K1, Sema L, SEMA7A, SEMAK1, SEMAL, Semaphorin 7A, Semaphorin K1, Semaphorin L |
| 计算分子量 | 666 aa, 75 kDa |
| 观测分子量 | 75-80 kDa |
| GenBank蛋白编号 | BC101643 |
| 基因名称 | SEMA7A |
| Gene ID (NCBI) | 8482 |
| RRID | AB_2878489 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75326 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SEMA7A, also named CD108 and SEMAL, belongs to the semaphorin family. SEMA7A is the only membrane‐associated glycosylphosphatidylinositol (GPI)‐linked semaphorin and plays a role in the regulation of neuronal axon outgrowth. In addition to its role in guiding axon pathfinding during neuronal development, Sema7A has diverse functions in morphogenesis and immune cell control and regulates T cell responses via the α1β1 integrin receptor (PMID: 31179640). SEMA7A has 2 isoforms with molecular weights of 73 and 75 kDa, respectively.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SEMA7A antibody 18070-1-AP | Download protocol |
| IP protocol for SEMA7A antibody 18070-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest EMT-activated secretory and endocytic vesicular trafficking programs underlie a vulnerability to PI4K2A antagonism in lung cancer | ||
Mol Metab Sema7A protects against high-fat diet-induced obesity and hepatic steatosis by regulating adipo/lipogenesis | ||
J Cancer NCAPG promotes the malignant progression of endometrioid cancer through LEF1/SEMA7A/PI3K-AKT |