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- KD/KO Validated
SENP1 Polyclonal antibody
SENP1 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HL-60 cells, HeLa cells, HepG2 cells, Jurkat cells, mouse testis tissue, rat testis tissue |
Positive IP detected in | Jurkat cells |
Positive IHC detected in | human ovary tissue, human liver tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
25349-1-AP targets SENP1 in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IP, IHC, ELISA Application Description |
Cited Applications | WB, IHC, CoIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | SENP1 fusion protein Ag18028 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | SUMO1/sentrin specific peptidase 1 |
Synonyms | SENP1, Sentrin specific protease 1, SuPr 2, SuPr2, SENP 1 |
Calculated Molecular Weight | 644 aa, 73 kDa |
Observed Molecular Weight | 73-74 kDa |
GenBank Accession Number | BC045639 |
Gene Symbol | SENP1 |
Gene ID (NCBI) | 29843 |
RRID | AB_2880039 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for SENP1 antibody 25349-1-AP | Download protocol |
IHC protocol for SENP1 antibody 25349-1-AP | Download protocol |
IP protocol for SENP1 antibody 25349-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Nature Lactate regulates cell cycle by remodeling the anaphase promoting complex
| ||
Free Radic Biol Med Sentrin-specific protease 1 maintains mitochondrial homeostasis through targeting the deSUMOylation of sirtuin-3 to alleviate oxidative damage induced by hepatic ischemia/reperfusion | ||
Int Immunopharmacol Nicotinamide mononucleotide attenuates airway epithelial barrier dysfunction via inhibiting SIRT3 SUMOylation in asthma | ||
Cancer Med Long noncoding RNA MCM3AP-AS1 enhances cell proliferation and metastasis in colorectal cancer by regulating miR-193a-5p/SENP1. | ||
J Pharmacol Sci Momordin Ic induces G0/1 phase arrest and apoptosis in colon cancer cells by suppressing SENP1/c-MYC signaling pathway.
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J Virol Multiple-Site SUMOylation of FMDV 3C Protease and Its Negative Role in Viral Replication
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