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SENP1 Recombinant antibody, PBS Only (Detector)

SENP1 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240478F3

Cat no : 83594-1-PBS

Print datasheet

Synonyms

SuPr2, SuPr 2, Sentrin specific protease 1, SENP 1, 240478F3

验证数据展示

  • IHC staining of human intrahepatic cholangiocarcinoma using 83594-1-RR (same clone as 83594-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 83594-1-RR (same clone as 83594-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83594-1-RR (SENP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83594-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 83594-1-RR (same clone as 83594-1-PBS)

    IF Staining of A431 using 83594-1-RR (same clone as 83594-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using SENP1 antibody (83594-1-RR, Clone: 240478F3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83594-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83594-1-RR (same clone as 83594-1-PBS)

    FC experiment of HeLa using 83594-1-RR (same clone as 83594-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SENP1 Recombinant antibody (83594-1-RR, Clone:240478F3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83594-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00607-1

    Cytometric bead array standard curve of MP00607-1

    Cytometric bead array standard curve of MP00607-1, SENP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83594-2-PBS. Detection antibody: 83594-1-PBS. Standard: Ag18028. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 83594-1-RR

    Affinity and Kinetic Characterization of 83594-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83594-1-RR against Human SENP1 were performed. The affinity constant is 1.91 nM.

产品信息

83594-1-PBS targets SENP1 as part of a matched antibody pair:

MP00607-1: 83594-2-PBS capture and 83594-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen SENP1 fusion protein Ag18028 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name SUMO1/sentrin specific peptidase 1
Synonyms SuPr2, SuPr 2, Sentrin specific protease 1, SENP 1, 240478F3
Calculated Molecular Weight 644 aa, 73 kDa
GenBank Accession NumberBC045639
Gene Symbol SENP1
Gene ID (NCBI) 29843
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9P0U3
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SENP1, Sentrin-specific protease 1, is a cysteine protease that specifically targets members of the small ubiquitin-like modifier (SUMO) protein family. SENP1 catalyzes two functions in the SUMO pathway (PMID: 29506078).The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins (PMID: 16253240). SENP1 is located in the nucleus and cytosol.