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SENP1 Recombinant antibody

SENP1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240478D3

Cat No : 83594-4-RR

Print datasheet

Synonyms

SENP1, Sentrin specific protease 1, SuPr 2

验证数据展示

  • WB analysis using 83594-4-RR

    WB analysis using 83594-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83594-4-RR (SENP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, HL-60 cells, Jurkat cells, rat testis tiuuse

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83594-4-RR is SENP1 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen SENP1 fusion protein Ag18028 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name SUMO1/sentrin specific peptidase 1
Synonyms SENP1, Sentrin specific protease 1, SuPr 2
Calculated Molecular Weight 644 aa, 73 kDa
Observed Molecular Weight73 kDa
GenBank Accession NumberBC045639
Gene Symbol SENP1
Gene ID (NCBI) 29843
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9P0U3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SENP1, Sentrin-specific protease 1, is a cysteine protease that specifically targets members of the small ubiquitin-like modifier (SUMO) protein family. SENP1 catalyzes two functions in the SUMO pathway (PMID: 29506078).The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins (PMID: 16253240). SENP1 is located in the nucleus and cytosol.

实验方案

Product Specific Protocols
WB protocol for SENP1 antibody 83594-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols