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验证数据展示

  • WB analysis using 83594-4-RR

    WB analysis using 83594-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83594-4-RR (SENP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovarian cancer using 83594-4-RR

    IHC staining of human ovarian cancer using 83594-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83594-4-RR (SENP1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83594-4-RR

    Affinity and Kinetic Characterization of 83594-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83594-4-RR against Human SENP1 were performed. The affinity constant is below 1 pM.

SENP1 Recombinant antibody

SENP1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83594-4-RR
CloneNo. 240478D3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

SuPr2, SuPr 2, Sentrin specific protease 1, SENP 1, 240478D3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HL-60 cells, Jurkat cells, rat testis tiuuse
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83594-4-RR targets SENP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SENP1 fusion protein Ag18028 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name SUMO1/sentrin specific peptidase 1
Synonyms SuPr2, SuPr 2, Sentrin specific protease 1, SENP 1, 240478D3
Calculated Molecular Weight 644 aa, 73 kDa
Observed Molecular Weight73 kDa
GenBank Accession NumberBC045639
Gene Symbol SENP1
Gene ID (NCBI) 29843
RRIDAB_3671210
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9P0U3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SENP1, Sentrin-specific protease 1, is a cysteine protease that specifically targets members of the small ubiquitin-like modifier (SUMO) protein family. SENP1 catalyzes two functions in the SUMO pathway (PMID: 29506078).The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins (PMID: 16253240). SENP1 is located in the nucleus and cytosol.

实验方案

Product Specific Protocols
WB protocol for SENP1 antibody 83594-4-RRDownload protocol
IHC protocol for SENP1 antibody 83594-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols