验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HL-60 cells, Jurkat cells, rat testis tiuuse |
Positive IHC detected in | human ovarian cancer Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83594-4-RR targets SENP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | SENP1 fusion protein Ag18028 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | SUMO1/sentrin specific peptidase 1 |
Synonyms | SuPr2, SuPr 2, Sentrin specific protease 1, SENP 1, 240478D3 |
Calculated Molecular Weight | 644 aa, 73 kDa |
Observed Molecular Weight | 73 kDa |
GenBank Accession Number | BC045639 |
Gene Symbol | SENP1 |
Gene ID (NCBI) | 29843 |
RRID | AB_3671210 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | Q9P0U3 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SENP1, Sentrin-specific protease 1, is a cysteine protease that specifically targets members of the small ubiquitin-like modifier (SUMO) protein family. SENP1 catalyzes two functions in the SUMO pathway (PMID: 29506078).The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins (PMID: 16253240). SENP1 is located in the nucleus and cytosol.
实验方案
Product Specific Protocols | |
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WB protocol for SENP1 antibody 83594-4-RR | Download protocol |
IHC protocol for SENP1 antibody 83594-4-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |