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SENP3 Polyclonal antibody
SENP3 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat and More (1)
Applications
WB, IHC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, Y79 cells, rat embryo tissue |
| Positive IP detected in | Y79 cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17659-1-AP targets SENP3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, rat samples.
| Tested Applications | WB, IHC, IP, ELISA Application Description |
| Cited Applications | WB, IF, IP |
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | SENP3 fusion protein Ag11911 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | SUMO1/sentrin/SMT3 specific peptidase 3 |
| Synonyms | SUMO-1-specific protease 3, SSP3, SMT3IP1, Sentrin-specific protease 3, Sentrin/SUMO-specific protease SENP3 |
| Calculated Molecular Weight | 574 aa, 65 kDa |
| Observed Molecular Weight | 65-70 kDa |
| GenBank Accession Number | BC080658 |
| Gene Symbol | SENP3 |
| Gene ID (NCBI) | 26168 |
| RRID | AB_2301618 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H4L4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SENP3(Sentrin-specific protease 3) is also named as SSP3, SUSP3 and belongs to the peptidase C48 family. It acts as an essential factor for ribosome biogenesis. SENP3 might regulate the sumoylation of other substrates through directly binding or mediating association with NPM1 at the nucleolus and other subcellular compartments.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SENP3 antibody 17659-1-AP | Download protocol |
| IHC protocol for SENP3 antibody 17659-1-AP | Download protocol |
| IP protocol for SENP3 antibody 17659-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Proteomics Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation. | ||
Int J Oncol Transcription factor E2F4 facilitates SUMOylation to promote HCC progression through interaction with LIN9 | ||
FASEB J SUMO1 SUMOylates and SENP3 deSUMOylates NLRP3 to orchestrate the inflammasome activation. | ||
Breast Cancer Res Treat Targeting PELP1 oncogenic signaling in TNBC with the small molecule inhibitor SMIP34 | ||
iScience SENP3-mediated deSUMOylation of c-Jun facilitates microglia-induced neuroinflammation after cerebral ischemia and reperfusion injury
| ||
Mol Oncol PELP1 inhibition by SMIP34 reduces endometrial cancer progression via attenuation of ribosomal biogenesis |
