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SENP8 Polyclonal antibody

SENP8 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13479-1-AP

Print datasheet

Synonyms

HsT17512, FKSG8, EC:3.4.22.-, Deneddylase-1, Deneddylase 1

验证数据展示

  • IHC staining of human colon cancer using 13479-1-AP

    IHC staining of human colon cancer using 13479-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SENP8 antibody included
  • IHC staining of human kidney using 13479-1-AP

    IHC staining of human kidney using 13479-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SENP8 antibody included
  • IHC staining of human stomach cancer using 13479-1-AP

    IHC staining of human stomach cancer using 13479-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SENP8 antibody included
  • IHC staining of mouse pancreas using 13479-1-AP

    IHC staining of mouse pancreas using 13479-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SENP8 antibody included
  • IHC staining of rat kidney using 13479-1-AP

    IHC staining of rat kidney using 13479-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SENP8 antibody included
  • IHC staining of rat pancreas using 13479-1-AP

    IHC staining of rat pancreas using 13479-1-AP

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SENP8 antibody included
  • IHC staining of mouse kidney using 13479-1-AP

    IHC staining of mouse kidney using 13479-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:1 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SENP8 antibody included
  • IHC staining of mouse kidney using 13479-1-AP

    IHC staining of mouse kidney using 13479-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:1 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SENP8 antibody included
  • IHC staining of human liver using 13479-1-AP

    IHC staining of human liver using 13479-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SENP8 antibody included
  • IF Staining of A431 using 13479-1-AP

    IF Staining of A431 using 13479-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using SENP8 antibody (13479-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

经过测试的应用

Positive IHC detected inrat kidney tissue, human liver tissue, human colon cancer tissue, human kidney tissue, human stomach cancer tissue, mouse pancreas tissue, rat pancreas tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy SENP8 Ready-To-Use IHC Kit. SENP8 primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13479-1-AP targets SENP8 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SENP8 fusion protein Ag4287 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SUMO/sentrin specific peptidase family member 8
Synonyms HsT17512, FKSG8, EC:3.4.22.-, Deneddylase-1, Deneddylase 1
Calculated Molecular Weight 212 aa, 24 kDa
Observed Molecular Weight24-28 kDa
GenBank Accession NumberBC031411
Gene Symbol SENP8
Gene ID (NCBI) 123228
RRIDAB_3085415
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96LD8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SENP8, a human NEDD8-specific protease, also known as NEDP1. It is expected to be located in cytoplasm and nucleus, which is highly expressed in kidney and pancreas. The calculated molecular weight of SENP8 is 24 kDa. It is a protease that catalyzes two essential functions in the NEDD8 pathway: processing of full-length NEDD8 to its mature form and deconjugation of NEDD8 from targeted proteins such as cullins or p53 (PMID: 15242646). A single-residue difference in the C-terminus of NEDD8 and ubiquitin contributes significantly to the ability of NEDP1 to discriminate between them. In vivo analysis indicates that NEDP1 mutants perturb deNEDDylation of the tumour suppressor p53 (PMID: 12759363).

实验方案

Product Specific Protocols
IHC protocol for SENP8 antibody 13479-1-APDownload protocol
IF protocol for SENP8 antibody 13479-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols