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验证数据展示

  • IHC staining of human colon cancer using 13479-1-AP

    IHC staining of human colon cancer using 13479-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SENP8 antibody included
  • IHC staining of human kidney using 13479-1-AP

    IHC staining of human kidney using 13479-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SENP8 antibody included
  • IHC staining of human stomach cancer using 13479-1-AP

    IHC staining of human stomach cancer using 13479-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SENP8 antibody included
  • IHC staining of mouse pancreas using 13479-1-AP

    IHC staining of mouse pancreas using 13479-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SENP8 antibody included
  • IHC staining of rat kidney using 13479-1-AP

    IHC staining of rat kidney using 13479-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SENP8 antibody included
  • IHC staining of rat pancreas using 13479-1-AP

    IHC staining of rat pancreas using 13479-1-AP

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SENP8 antibody included
  • IHC staining of mouse kidney using 13479-1-AP

    IHC staining of mouse kidney using 13479-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:1 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SENP8 antibody included
  • IHC staining of mouse kidney using 13479-1-AP

    IHC staining of mouse kidney using 13479-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:1 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SENP8 antibody included
  • IHC staining of human liver using 13479-1-AP

    IHC staining of human liver using 13479-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SENP8 antibody included
  • IF Staining of A431 using 13479-1-AP

    IF Staining of A431 using 13479-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using SENP8 antibody (13479-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

SENP8 Polyclonal antibody

SENP8 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 13479-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF/ICC, ELISA

别名

HsT17512, FKSG8, EC:3.4.22.-, Deneddylase-1, Deneddylase 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inrat kidney tissue, human liver tissue, human colon cancer tissue, human kidney tissue, human stomach cancer tissue, mouse pancreas tissue, rat pancreas tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy SENP8 Ready-To-Use IHC Kit. SENP8 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13479-1-AP targets SENP8 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SENP8 fusion protein Ag4287 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SUMO/sentrin specific peptidase family member 8
别名 HsT17512, FKSG8, EC:3.4.22.-, Deneddylase-1, Deneddylase 1
计算分子量 212 aa, 24 kDa
观测分子量24-28 kDa
GenBank蛋白编号BC031411
基因名称 SENP8
Gene ID (NCBI) 123228
RRIDAB_3085415
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96LD8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SENP8, a human NEDD8-specific protease, also known as NEDP1. It is expected to be located in cytoplasm and nucleus, which is highly expressed in kidney and pancreas. The calculated molecular weight of SENP8 is 24 kDa. It is a protease that catalyzes two essential functions in the NEDD8 pathway: processing of full-length NEDD8 to its mature form and deconjugation of NEDD8 from targeted proteins such as cullins or p53 (PMID: 15242646). A single-residue difference in the C-terminus of NEDD8 and ubiquitin contributes significantly to the ability of NEDP1 to discriminate between them. In vivo analysis indicates that NEDP1 mutants perturb deNEDDylation of the tumour suppressor p53 (PMID: 12759363).

实验方案

Product Specific Protocols
IHC protocol for SENP8 antibody 13479-1-APDownload protocol
IF protocol for SENP8 antibody 13479-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols