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  • Featured Product
  • KD/KO Validated

SEPHS1 Polyclonal antibody

SEPHS1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16635-1-AP

Print datasheet

Synonyms

SELD, Selenide, water dikinase 1, Selenium donor protein 1, Selenophosphate synthase 1, selenophosphate synthetase 1, SEPHS1, SPS, SPS1

验证数据展示

  • WB analysis of HepG2 using 16635-1-AP

    WB analysis of HepG2 using 16635-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16635-1-AP (SEPHS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis using 16635-1-AP

    WB analysis using 16635-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16635-1-AP (SEPHS1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 16635-1-AP

    IHC staining of human lung cancer using 16635-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 16635-1-AP (SEPHS1 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with SEPHS1 antibody included
  • IHC staining of human lung cancer using 16635-1-AP

    IHC staining of human lung cancer using 16635-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 16635-1-AP (SEPHS1 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with SEPHS1 antibody included

经过测试的应用

Positive WB detected inJurkat cells, HepG2 cells, mouse liver tissue, T-47D cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SEPHS1 Ready-To-Use IHC Kit. SEPHS1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16635-1-AP targets SEPHS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen SEPHS1 fusion protein Ag9978 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name selenophosphate synthetase 1
Synonyms SELD, Selenide, water dikinase 1, Selenium donor protein 1, Selenophosphate synthase 1, selenophosphate synthetase 1, SEPHS1, SPS, SPS1
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC000941
Gene Symbol SEPHS1
Gene ID (NCBI) 22929
RRIDAB_10603472
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49903
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Selenophosphate synthetase 1 (SEPHS1) has been implicated in the maintenance of redox homeostasis in chondrocytes and its deficiency plays a causal role in the progression of osteoarthritis (PMID: 36202216). SEPHS1 downregulation in human umbilical vein cells (HUVECs) led to cell death rather than the inhibition of cell proliferation (PMID: 33622411).

实验方案

Product Specific Protocols
WB protocol for SEPHS1 antibody 16635-1-APDownload protocol
IHC protocol for SEPHS1 antibody 16635-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Biomaterials

H2Se-evolving bio-heterojunctions promote cutaneous regeneration in infected wounds by inhibiting excessive cellular senescence

Authors - Fan Yang
View Article
humanWB,IHC

Exp Hematol Oncol

SEPHS1 promotes SMAD2/3/4 expression and hepatocellular carcinoma cells invasion.

Authors - Shu Yang
  • KD Validated
View Article