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验证数据展示

  • WB analysis using 82284-1-RR (same clone as 82284-1-PBS)

    WB analysis using 82284-1-RR (same clone as 82284-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82284-1-RR (SEPT4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82284-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 82284-1-RR (same clone as 82284-1-PBS)

    IHC staining of mouse brain using 82284-1-RR (same clone as 82284-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82284-1-RR (SEPT4 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82284-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SEPT4 antibody included
  • IHC staining of mouse brain using 82284-1-RR (same clone as 82284-1-PBS)

    IHC staining of mouse brain using 82284-1-RR (same clone as 82284-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82284-1-RR (SEPT4 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82284-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SEPT4 antibody included
  • Affinity and Kinetic Characterization of 82284-1-RR

    Affinity and Kinetic Characterization of 82284-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82284-1-RR against Human SEPT4 were performed. The affinity constant is 1.38 nM.

SEPT4 Recombinant antibody, PBS Only

SEPT4 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 82284-1-PBS

产品说明书

CloneNo. 5O8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

hCDCREL-2, hCDCREL2, Cell division control-related protein 2, C17orf47, BRADEION

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SEPT4 Ready-To-Use IHC Kit. SEPT4 primary antibody included.

产品信息

82284-1-PBS targets SEPT4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SEPT4 fusion protein Ag3185 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 septin 4
别名 hCDCREL-2, hCDCREL2, Cell division control-related protein 2, C17orf47, BRADEION
计算分子量 459 aa, 53 kDa
观测分子量38-55 kDa
GenBank蛋白编号BC018056
基因名称 SEPT4
Gene ID (NCBI) 5414
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO43236
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Spetin 4 is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Spetin 4 was widely expressed in brain and heart, also expressed in colorectal cancers and malignant melanomas. This protein has been implicated in diverse cellular functions, including cytokinesis, apoptosis, and tumor suppression(PMID:15737931). There are several isoforms of SEPTIN4, four of which were detected in the mouse brain and mouse cerebellum, 54 kDa, 52 kDa, 48 kDa and 44 kDa respectively(PMID: 23938054; PMID: 26657011).