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Septin 6 Polyclonal antibody

Septin 6 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 12805-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA and More (1)

KIAA0128, SEP2, SEPT2, SEPT6, septin 6, septin6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inJurkat cells, K-562 cells, rat brain tissue
Positive IP detected inrat brain tissue
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SEPT6 Ready-To-Use IHC Kit. SEPT6 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12805-1-AP targets Septin 6 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 Septin 6 fusion protein Ag3521 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 septin 6
别名 KIAA0128, SEP2, SEPT2, SEPT6, septin 6, septin6
计算分子量 434 aa, 50 kDa
观测分子量 50 kDa
GenBank蛋白编号BC036240
基因名称 SEPT6
Gene ID (NCBI) 23157
RRIDAB_2184993
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14141
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Septin 6 belongs to the septins family which are conserved GTP-binding proteins functioning as dynamic, regulatable scaffolds for the recruitment of other proteins. They are involved in membrane dynamics, vesicle trafficking, apoptosis, and cytoskeletal remodeling. Septin 6 also interacts with hepatitis C virus (HCV) NS5b RNA polymerase and NS5b-binding protein ( HNRNPA1) and may play a role in HCV RNA replication.

实验方案

Product Specific Protocols
WB protocol for Septin 6 antibody 12805-1-APDownload protocol
IHC protocol for Septin 6 antibody 12805-1-APDownload protocol
IP protocol for Septin 6 antibody 12805-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Biol Chem

Uncovering principles that control septin-septin interactions.

Authors - Moshe S Kim
human,ratWB

Lab Invest

Effect of SEPT6 on the biological behavior of hepatic stellate cells and liver fibrosis in rats and its mechanism.

Authors - Yuhui Fan
  • KD Validated
mouseWB

Proteomics

Quantification of protein isoforms in mesenchymal stem cells by reductive dimethylation of lysines in intact proteins.

Authors - She Yi-Min YM
humanIHC

Int J Oncol

SEPT6 drives hepatocellular carcinoma cell proliferation, migration and invasion via the Hippo/YAP signaling pathway.

Authors - Yuhui Fan
humanWB

Mol Biol Cell

Revised subunit order of mammalian septin complexes explains their in vitro polymerization properties.

Authors - Forooz Soroor
humanIF

Mol Hum Reprod

The SEPT12 complex is required for the establishment of a functional sperm head-tail junction.

Authors - Yi-Ru Shen
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