验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, K-562 cells, rat brain tissue |
| Positive IP detected in | rat brain tissue |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy SEPT6 Ready-To-Use IHC Kit. SEPT6 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12805-1-AP targets Septin 6 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | Septin 6 fusion protein Ag3521 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | septin 6 |
| Synonyms | KIAA0128, SEP2, SEPT2, SEPT6, septin 6, septin6 |
| Calculated Molecular Weight | 434 aa, 50 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC036240 |
| Gene Symbol | SEPT6 |
| Gene ID (NCBI) | 23157 |
| RRID | AB_2184993 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14141 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Septin 6 belongs to the septins family which are conserved GTP-binding proteins functioning as dynamic, regulatable scaffolds for the recruitment of other proteins. They are involved in membrane dynamics, vesicle trafficking, apoptosis, and cytoskeletal remodeling. Septin 6 also interacts with hepatitis C virus (HCV) NS5b RNA polymerase and NS5b-binding protein ( HNRNPA1) and may play a role in HCV RNA replication.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Septin 6 antibody 12805-1-AP | Download protocol |
| IHC protocol for Septin 6 antibody 12805-1-AP | Download protocol |
| IP protocol for Septin 6 antibody 12805-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Lab Invest Effect of SEPT6 on the biological behavior of hepatic stellate cells and liver fibrosis in rats and its mechanism.
| ||
Proteomics Quantification of protein isoforms in mesenchymal stem cells by reductive dimethylation of lysines in intact proteins. | ||
Int J Oncol SEPT6 drives hepatocellular carcinoma cell proliferation, migration and invasion via the Hippo/YAP signaling pathway. | ||
Mol Biol Cell Revised subunit order of mammalian septin complexes explains their in vitro polymerization properties. | ||
Mol Hum Reprod The SEPT12 complex is required for the establishment of a functional sperm head-tail junction. |
