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Septin 8 Polyclonal antibody

Septin 8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11769-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

SEPT8, Septin-8, SEPTIN8, SEP2, KIAA0202

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, mouse testis tissue, K-562 cells, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman gliomas tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11769-1-AP targets Septin 8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 Septin 8 fusion protein Ag2357 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 septin 8
别名 SEPT8, Septin-8, SEPTIN8, SEP2, KIAA0202
计算分子量55.6 kDa
观测分子量 50-56 kDa
GenBank蛋白编号BC001329
基因名称 Septin 8
Gene ID (NCBI) 23176
RRIDAB_2185006
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92599
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Septin 8, also named as KIAA0202, is a member of the highly conserved septin family. Septins are 40-60kD GTPases that assemble as filamentous scaffolds. They are involved in the organization of submembranous structures, in neuronal polarity, and in vesicle trafficking. It may play a role in platelet secretion. Septin 8 is about 50-56 kDa in WB detection.

实验方案

Product Specific Protocols
WB protocol for Septin 8 antibody 11769-1-APDownload protocol
IHC protocol for Septin 8 antibody 11769-1-APDownload protocol
IF protocol for Septin 8 antibody 11769-1-APDownload protocol
IP protocol for Septin 8 antibody 11769-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Elife

Septin/anillin filaments scaffold central nervous system myelin to accelerate nerve conduction.

Authors - Julia Patzig
mouseWB,IF

Thromb Haemost

Impaired Platelet Function in Sept8-Deficient Mice In Vitro

Authors - Katharina Neubauer
mouseWB

Glia

Developmental maturation and regional heterogeneity but no sexual dimorphism of the murine CNS myelin proteome

Authors - Sophie B Siems

Glia

The formation of paranodal spirals at the ends of CNS myelin sheaths requires the planar polarity protein Vangl2.

Authors - Andrew A Jarjour
mouseWB

Development

Pinch2 regulates myelination in the mouse central nervous system.

Authors - Joana Paes de Faria
mouse, humanWB

J Cell Sci

SEPT8 modulates β-amyloidogenic processing of APP by affecting the sorting and accumulation of BACE1.

Authors - Kaisa M A Kurkinen
  • KD Validated
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