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SERAC1 Polyclonal antibody

SERAC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25729-1-AP

Print datasheet

Synonyms

Protein SERAC1, SERAC1

验证数据展示

  • WB analysis of mouse brain using 25729-1-AP

    WB analysis of mouse brain using 25729-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25729-1-AP (SERAC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 25729-1-AP

    IHC staining of human colon using 25729-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25729-1-AP (SERAC1 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 25729-1-AP

    IF Staining of HepG2 using 25729-1-AP

    Immunofluorescent analysis of HepG2 cells using 25729-1-AP (SERAC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse brain tissue, HEK-293 cells, HeLa cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25729-1-AP is SERAC1 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen SERAC1 fusion protein Ag22790 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name serine active site containing 1
Synonyms Protein SERAC1, SERAC1
Calculated Molecular Weight 654 aa, 74 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC001705
Gene Symbol SERAC1
Gene ID (NCBI) 84947
RRIDAB_2880213
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96JX3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SERAC1 antibody 25729-1-APDownload protocol
IHC protocol for SERAC1 antibody 25729-1-APDownload protocol
IF protocol for SERAC1 antibody 25729-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols