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验证数据展示

  • WB analysis of HEK-293 using 67248-1-Ig (same clone as 67248-1-PBS)

    WB analysis of HEK-293 using 67248-1-Ig (same clone as 67248-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67248-1-PBS in a different storage buffer formulation.

  • IHC staining of human urothelial carcinoma using 67248-1-Ig (same clone as 67248-1-PBS)

    IHC staining of human urothelial carcinoma using 67248-1-Ig (same clone as 67248-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67248-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ATP2A2 antibody included
  • IHC staining of human urothelial carcinoma using 67248-1-Ig (same clone as 67248-1-PBS)

    IHC staining of human urothelial carcinoma using 67248-1-Ig (same clone as 67248-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67248-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ATP2A2 antibody included
  • IHC staining of mouse skeletal muscle using 67248-1-Ig (same clone as 67248-1-PBS)

    IHC staining of mouse skeletal muscle using 67248-1-Ig (same clone as 67248-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67248-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ATP2A2 antibody included
  • IHC staining of mouse skeletal muscle using 67248-1-Ig (same clone as 67248-1-PBS)

    IHC staining of mouse skeletal muscle using 67248-1-Ig (same clone as 67248-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67248-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ATP2A2 antibody included

SERCA2,ATP2A2 Monoclonal antibody, PBS Only

SERCA2,ATP2A2 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67248-1-PBS

产品说明书

CloneNo. 1F11A11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

ATP2A2, SERCA2, SERCA2b, Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform, Calcium pump 2

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ATP2A2 Ready-To-Use IHC Kit. ATP2A2 primary antibody included.

产品信息

67248-1-PBS targets SERCA2,ATP2A2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SERCA2,ATP2A2 fusion protein Ag5052 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
别名 ATP2A2, SERCA2, SERCA2b, Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform, Calcium pump 2
计算分子量 1042 aa, 115 kDa
观测分子量 120 kDa
GenBank蛋白编号BC035588
基因名称 SERCA2
Gene ID (NCBI) 488
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP16615
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATP2A2, also known as SERCA2 (Sarco/endoplasmic reticulum-type calcium transport ATPase 2), is a 110 kDa magnesium-dependent enzyme catalyzing the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Human SERCA2 gene gives rise to several alternatively spliced isoforms: SERCA2a, SERCA2b and SERCA2c. ATP2A2 is mainly expressed in the sarcoplasmic reticulum of muscle cells and the endoplasmic reticulum of most cell types.