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SERPINA10 Polyclonal antibody

SERPINA10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat No : 11851-1-AP

Print datasheet

Synonyms

ZPI, UNQ707/PRO1358, Serpin A10, PZI, PZ-dependent protease inhibitor

验证数据展示

  • WB analysis of human liver using 11851-1-AP

    WB analysis of human liver using 11851-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of L02 using 11851-1-AP

    IP experiment of L02 using 11851-1-AP

    IP result of anti-SERPINA10 (IP:11851-1-AP, 3ug; Detection:11851-1-AP 1:1000) with L02 cells lysate 2500ug.

  • IHC staining of human liver using 11851-1-AP

    IHC staining of human liver using 11851-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 11851-1-AP (SERPINA10 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human liver using 11851-1-AP

    IHC staining of human liver using 11851-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 11851-1-AP (SERPINA10 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of L02 using 11851-1-AP

    IF Staining of L02 using 11851-1-AP

    Immunofluorescent analysis of L02 cells using 11851-1-AP (SERPINA10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman liver tissue, HepG2 cells, L02 cells, mouse liver tissue, RAW264.7
Positive IP detected inL02 cells
Positive IHC detected inhuman liver tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inL02 cells, HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11851-1-AP targets SERPINA10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SERPINA10 fusion protein Ag2420 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 10
Synonyms ZPI, UNQ707/PRO1358, Serpin A10, PZI, PZ-dependent protease inhibitor
Calculated Molecular Weight 444 aa, 51 kDa
Observed Molecular Weight 72 kDa, 68 kDa, 50-55 kDa
GenBank Accession NumberBC022261
Gene Symbol SERPINA10
Gene ID (NCBI) 51156
RRIDAB_2254343
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UK55
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SERPINA10 antibody 11851-1-APDownload protocol
IHC protocol for SERPINA10 antibody 11851-1-APDownload protocol
IF protocol for SERPINA10 antibody 11851-1-APDownload protocol
IP protocol for SERPINA10 antibody 11851-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols