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Kallistatin Polyclonal antibody

Kallistatin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 11430-2-AP

Print datasheet

Synonyms

Serpin A4, PI 4, Peptidase inhibitor 4, KST, KLST

验证数据展示

  • WB analysis of human spleen using 11430-2-AP

    WB analysis of human spleen using 11430-2-AP

    human spleen tissue were subjected to SDS PAGE followed by western blot with 11430-2-AP (Kallistatin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 11430-2-AP

    IHC staining of human pancreas cancer using 11430-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11430-2-AP (Kallistatin antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of HCT 116 using 11430-2-AP

    IF Staining of HCT 116 using 11430-2-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using Kallistatin antibody (11430-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inhuman spleen tissue, human plasma tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHCT 116 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11430-2-AP targets Kallistatin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivitymouse
Immunogen Kallistatin fusion protein Ag1962 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4
Synonyms Serpin A4, PI 4, Peptidase inhibitor 4, KST, KLST
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC014992
Gene Symbol Kallistatin
Gene ID (NCBI) 5267
RRIDAB_2185735
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP29622
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Kallistatin antibody 11430-2-APDownload protocol
IHC protocol for Kallistatin antibody 11430-2-APDownload protocol
IF protocol for Kallistatin antibody 11430-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Diabetes

Pathogenic Role of Diabetes-induced Overexpression of Kallistatin in Corneal Wound Healing Deficiency through Inhibition of Canonical Wnt Signaling.

Authors - Wentao Liang
View Article