验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, A549 cells, HeLa cells, Jurkat cells, L02 cells, PC-3 cells |
| Positive IHC detected in | human ovary tumor tissue, human breast cancer tissue, human ovary cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:600 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10673-1-AP targets Protein C inhibitor in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, CoIP, ELISA |
| 经测试反应性 | human |
| 文献引用反应性 | human, rat |
| 免疫原 | Protein C inhibitor fusion protein Ag1078 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5 |
| 别名 | Serpin A5, PROCI, Plasminogen activator inhibitor 3, Plasma serine protease inhibitor, PLANH3 |
| 计算分子量 | 46 kDa |
| 观测分子量 | 46 kDa |
| GenBank蛋白编号 | BC008915 |
| 基因名称 | SERPINA5 |
| Gene ID (NCBI) | 5104 |
| RRID | AB_2185741 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P05154 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SERPINA5 (also called Protein C Inhibitor, PCI ) is a serpin type of serine protease inhibitor that is found in many tissues and fluids in human, including blood plasma, seminal plasma and urine. PCI found in blood originates from the liver and is capable of inhibiting several serine proteases involved in the regulation of coagulation and fibrinolysis, including activated protein C, thrombin, factor Xa, various kallikreins and plasminogen activators. PCI has been found to have antimicrobial and antitumor properties and thus appears to be a multi-functional protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Protein C inhibitor antibody 10673-1-AP | Download protocol |
| IHC protocol for Protein C inhibitor antibody 10673-1-AP | Download protocol |
| IF protocol for Protein C inhibitor antibody 10673-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mod Pathol Loss of SerpinA5 protein expression is associated with advanced-stage serous ovarian tumors. | ||
J Neuropathol Exp Neurol Gene expression profiling of NF-1-associated and sporadic pilocytic astrocytoma identifies aldehyde dehydrogenase 1 family member L1 (ALDH1L1) as an underexpressed candidate biomarker in aggressive subtypes. | ||
Proteomics Clin Appl Proteomic analysis of human articular cartilage unravels the dyscoagulation in osteoarthritis and the potential value of serpinA5 as a biomarker for osteoarthritis. | ||
J Cell Mol Med SERPINA5 promotes tumour cell proliferation by modulating the PI3K/AKT/mTOR signalling pathway in gastric cancer | ||
Biomolecules Improvement in Clinical Features of L-NAME-Induced Preeclampsia-like Rats through Reduced SERPINA5 Expression | ||
J Proteome Res Proteomics Analysis of Knee Subchondral Bone Identifies Differentially Expressed Proteins Associated with Osteoarthritis |
