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Protein C inhibitor Polyclonal antibody

Protein C inhibitor Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 10673-1-AP

Print datasheet

Synonyms

Serpin A5, PROCI, Plasminogen activator inhibitor 3, Plasma serine protease inhibitor, PLANH3

验证数据展示

  • WB analysis of HepG2 using 10673-1-AP

    WB analysis of HepG2 using 10673-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 10673-1-AP

    IHC staining of human ovary tumor using 10673-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10673-1-AP (Protein C inhibitor antibody at dilution of 1:50 (under 10x lens).

  • IHC staining of human ovary tumor using 10673-1-AP

    IHC staining of human ovary tumor using 10673-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10673-1-AP (Protein C inhibitor antibody at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 10673-1-AP

    IF Staining of HepG2 using 10673-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, A549 cells, HeLa cells, Jurkat cells, L02 cells, PC-3 cells
Positive IHC detected inhuman ovary tumor tissue, human breast cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:600
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10673-1-AP targets Protein C inhibitor in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, CoIP, ELISA
Tested Reactivity human
Cited Reactivityhuman, rat
Immunogen Protein C inhibitor fusion protein Ag1078 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5
Synonyms Serpin A5, PROCI, Plasminogen activator inhibitor 3, Plasma serine protease inhibitor, PLANH3
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC008915
Gene Symbol SERPINA5
Gene ID (NCBI) 5104
RRIDAB_2185741
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05154
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SERPINA5 (also called Protein C Inhibitor, PCI ) is a serpin type of serine protease inhibitor that is found in many tissues and fluids in human, including blood plasma, seminal plasma and urine. PCI found in blood originates from the liver and is capable of inhibiting several serine proteases involved in the regulation of coagulation and fibrinolysis, including activated protein C, thrombin, factor Xa, various kallikreins and plasminogen activators. PCI has been found to have antimicrobial and antitumor properties and thus appears to be a multi-functional protein.

实验方案

Product Specific Protocols
WB protocol for Protein C inhibitor antibody 10673-1-APDownload protocol
IHC protocol for Protein C inhibitor antibody 10673-1-APDownload protocol
IF protocol for Protein C inhibitor antibody 10673-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Mod Pathol

Loss of SerpinA5 protein expression is associated with advanced-stage serous ovarian tumors.

Authors - Bijsmans Ingrid T G W IT
View Article
humanIHC

J Neuropathol Exp Neurol

Gene expression profiling of NF-1-associated and sporadic pilocytic astrocytoma identifies aldehyde dehydrogenase 1 family member L1 (ALDH1L1) as an underexpressed candidate biomarker in aggressive subtypes.

Authors - Rodriguez Fausto J FJ
View Article
ratIHC

Proteomics Clin Appl

Proteomic analysis of human articular cartilage unravels the dyscoagulation in osteoarthritis and the potential value of serpinA5 as a biomarker for osteoarthritis.

Authors - Tengjiao Zhu
View Article
humanWB,CoIP

J Cell Mol Med

SERPINA5 promotes tumour cell proliferation by modulating the PI3K/AKT/mTOR signalling pathway in gastric cancer

Authors - Meiyang Fan
View Article
ratELISA,IHC

Biomolecules

Improvement in Clinical Features of L-NAME-Induced Preeclampsia-like Rats through Reduced SERPINA5 Expression

Authors - Shanshui Zeng
View Article
human,ratIHC

J Proteome Res

Proteomics Analysis of Knee Subchondral Bone Identifies Differentially Expressed Proteins Associated with Osteoarthritis

Authors - Qizhao Tan
View Article