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验证数据展示

  • WB analysis of human testis using 66030-1-Ig

    WB analysis of human testis using 66030-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 66030-1-Ig (Protein C inhibitor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 66030-1-Ig

    IHC staining of human ovary tumor using 66030-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66030-1-Ig (Protein C inhibitor antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 66030-1-Ig

    IHC staining of human ovary tumor using 66030-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66030-1-Ig (Protein C inhibitor antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human ovary tumor using 66030-1-Ig

    IF Staining of human ovary tumor using 66030-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using Protein C inhibitor antibody (66030-1-Ig, Clone: 2H6G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of human ovary tumor using 66030-1-Ig

    IF Staining of human ovary tumor using 66030-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using Protein C inhibitor antibody (66030-1-Ig, Clone: 2H6G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Protein C inhibitor Monoclonal antibody

Protein C inhibitor Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66030-1-Ig

产品说明书

CloneNo. 2H6G2

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF-P, ELISA

别名

PAI3, PCI, PLANH3, PROCI, Protein C inhibitor, SERPINA5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHuman testis, tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman ovary tumor tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66030-1-Ig targets Protein C inhibitor in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human
免疫原 Protein C inhibitor fusion protein Ag18094 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5
别名 PAI3, PCI, PLANH3, PROCI, Protein C inhibitor, SERPINA5
计算分子量 46 kDa
观测分子量 46 kDa
GenBank蛋白编号BC008915
基因名称 SERPINA5
Gene ID (NCBI) 5104
RRIDAB_11045661
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP05154
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SERPINA5 (also called Protein C Inhibitor, PCI ) is a serpin type of serine protease inhibitor that is found in many tissues and fluids in human, including blood plasma, seminal plasma and urine. PCI found in blood originates from the liver and is capable of inhibiting several serine proteases involved in the regulation of coagulation and fibrinolysis, including activated protein C, thrombin, factor Xa, various kallikreins and plasminogen activators. PCI has been found to have antimicrobial and antitumor properties and thus appears to be a multi-functional protein.

实验方案

Product Specific Protocols
WB protocol for Protein C inhibitor antibody 66030-1-IgDownload protocol
IHC protocol for Protein C inhibitor antibody 66030-1-IgDownload protocol
IF protocol for Protein C inhibitor antibody 66030-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols