验证数据展示
经过测试的应用
Positive WB detected in | human plasma, human urine |
Positive IP detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
16536-1-AP targets SERPINA7/TBG in WB, IP, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IP, ELISA Application Description |
经测试反应性 | human |
免疫原 | SERPINA7/TBG fusion protein Ag9823 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7 |
别名 | Serpin A7, SERPINA7, T4 binding globulin, TBG, Thyroxine binding globulin |
计算分子量 | 415 aa, 46 kDa |
观测分子量 | 54 kDa |
GenBank蛋白编号 | BC020747 |
基因名称 | SERPINA7 |
Gene ID (NCBI) | 6906 |
RRID | AB_3085495 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P05543 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SERPINA7, also known as Thyroxine-binding globulin (TBG), is a 54 kDa acidic glycoprotein, consisting of a single polypeptide chain of 395 amino acids and four oligosaccharide chains. It is one of the major serum transport proteins of thyroid hormones (THs). Although high amounts of albumin are found in serum, TBG has a greater affinity for thyroxine (T4). Mutations in this gene result in TGB deficiency, which has been classified as partial deficiency, complete deficiency, and excess, based on the level of serum TBG.
实验方案
Product Specific Protocols | |
---|---|
WB protocol for SERPINA7/TBG antibody 16536-1-AP | Download protocol |
IP protocol for SERPINA7/TBG antibody 16536-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |