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验证数据展示

  • WB analysis using 16536-1-AP

    WB analysis using 16536-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16536-1-AP (SERPINA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 16536-1-AP

    IP experiment of HepG2 using 16536-1-AP

    IP result of anti-SERPINA7/TBG (IP:16536-1-AP, 4ug; Detection:16536-1-AP 1:500) with HepG2 cells lysate 1080 ug.

SERPINA7/TBG Polyclonal antibody

SERPINA7/TBG Polyclonal Antibody for WB, IP, ELISA
Cat No. 16536-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

Serpin A7, SERPINA7, T4 binding globulin, TBG, Thyroxine binding globulin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inhuman plasma, human urine
Positive IP detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16536-1-AP targets SERPINA7/TBG in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human
Immunogen SERPINA7/TBG fusion protein Ag9823 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7
Synonyms Serpin A7, SERPINA7, T4 binding globulin, TBG, Thyroxine binding globulin
Calculated Molecular Weight 415 aa, 46 kDa
Observed Molecular Weight54 kDa
GenBank Accession NumberBC020747
Gene Symbol SERPINA7
Gene ID (NCBI) 6906
RRIDAB_3085495
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05543
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SERPINA7, also known as Thyroxine-binding globulin (TBG), is a 54 kDa acidic glycoprotein, consisting of a single polypeptide chain of 395 amino acids and four oligosaccharide chains. It is one of the major serum transport proteins of thyroid hormones (THs). Although high amounts of albumin are found in serum, TBG has a greater affinity for thyroxine (T4). Mutations in this gene result in TGB deficiency, which has been classified as partial deficiency, complete deficiency, and excess, based on the level of serum TBG.

实验方案

Product Specific Protocols
WB protocol for SERPINA7/TBG antibody 16536-1-APDownload protocol
IP protocol for SERPINA7/TBG antibody 16536-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols