• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

SERPINA7 Monoclonal antibody

SERPINA7 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1B11B10

Cat no : 66454-1-Ig

Print datasheet

Synonyms

Serpin A7, SERPINA7, T4 binding globulin, TBG, Thyroxine binding globulin

验证数据展示

  • WB analysis of human plasma using 66454-1-Ig

    WB analysis of human plasma using 66454-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66454-1-Ig (SERPINA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 66454-1-Ig

    IHC staining of human liver cancer using 66454-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66454-1-Ig (SERPINA7 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 66454-1-Ig

    IHC staining of human liver cancer using 66454-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66454-1-Ig (SERPINA7 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human liver cancer using 66454-1-Ig

    IF Staining of human liver cancer using 66454-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using SERPINA7 antibody (66454-1-Ig, Clone: 1B11B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of human liver cancer using 66454-1-Ig

    IF Staining of human liver cancer using 66454-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using SERPINA7 antibody (66454-1-Ig, Clone: 1B11B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inhuman plasma tissue, human testis tissue
Positive IHC detected inhuman liver cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

66454-1-Ig targets SERPINA7 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, pig, rat
Cited Reactivityhuman
Immunogen SERPINA7 fusion protein Ag9823 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7
Synonyms Serpin A7, SERPINA7, T4 binding globulin, TBG, Thyroxine binding globulin
Calculated Molecular Weight 415 aa, 46 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC020747
Gene Symbol SERPINA7
Gene ID (NCBI) 6906
RRIDAB_2881823
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05543
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SERPINA7 antibody 66454-1-IgDownload protocol
IHC protocol for SERPINA7 antibody 66454-1-IgDownload protocol
IF protocol for SERPINA7 antibody 66454-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Heliyon

Secreted proteins in plasma and placenta as novel non-invasive biomarkers for intrahepatic cholestasis of pregnancy: A case-control study

Authors - Lingyan Chen
View Article